title: Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.
creator: Bains, Jagmohan Singh
creator: Dhuna, Vikram
creator: Singh, Jatinder
creator: Kamboj, Sukhdev Singh
creator: Nijjar, Kamaljeet Kaur
creator: Agrewala, J N
subject: QR180 Immunology
description: Two novel lectins were purified from rhizomes of two sweet flag species, namely Acorus calamus (Linn.) and Acorus gramineus (Solandin Ait.) by affinity chromatography on mannose linked epoxy-activated Sepharose 6B. The apparent molecular mass of the lectins, as determined by gel filtration chromatography, was 56 kDa for ACL and 55 kDa for AGL. In SDS-PAGE, pH 8.3, both lectins migrated with a subunit molecular mass of 13.6 kDa and 13.5 kDa, respectively, under reducing and non-reducing conditions thus indicating the absence of disulphide linkages. Acorus lectins readily agglutinated rabbit, rat and guinea pig erythrocytes. Both ACL and AGL also reacted with RBCs from sheep, goat and human ABO blood groups after neuraminidase treatment. ACL and AGL were inhibited by mannose/glucose and their derivatives. The most effective inhibitor was methyl-alpha-D-mannopyranoside. Acorus lectins were stable up to 55 degrees C, did not require metal ions for their activity and were also affected by high concentrations of denaturants like urea, thiourea and guanidine-HCl. These lectins showed potent mitogenic activity towards mouse splenocytes and human lymphocytes. Both ACL and AGL also significantly inhibited the growth of J774, a murine macrophage cancer cell-line and to lesser extent WEHI-279, a B-cell lymphoma.
publisher: Elsevier Science
date: 2005-08
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/993/1/agrewala2005.pdf
relation: http://www.sciencedirect.com/science/article/pii/S1567576905001025
identifier:   Bains, Jagmohan Singh and Dhuna, Vikram and Singh, Jatinder and Kamboj, Sukhdev Singh and Nijjar, Kamaljeet Kaur and Agrewala, J N  (2005) Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.  International immunopharmacology, 5 (9).  pp. 1470-8.  ISSN 1567-5769     
relation: http://crdd.osdd.net/open/993/