creators_name: Bains, Jagmohan Singh
creators_name: Dhuna, Vikram
creators_name: Singh, Jatinder
creators_name: Kamboj, Sukhdev Singh
creators_name: Nijjar, Kamaljeet Kaur
creators_name: Agrewala, J N
type: article
datestamp: 2012-02-15 14:37:56
lastmod: 2015-01-07 05:22:28
metadata_visibility: show
title: Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.
ispublished: pub
subjects: QR180
full_text_status: restricted
note: Copyright of this article belongs to Elseveir Science.
abstract: Two novel lectins were purified from rhizomes of two sweet flag species, namely Acorus calamus (Linn.) and Acorus gramineus (Solandin Ait.) by affinity chromatography on mannose linked epoxy-activated Sepharose 6B. The apparent molecular mass of the lectins, as determined by gel filtration chromatography, was 56 kDa for ACL and 55 kDa for AGL. In SDS-PAGE, pH 8.3, both lectins migrated with a subunit molecular mass of 13.6 kDa and 13.5 kDa, respectively, under reducing and non-reducing conditions thus indicating the absence of disulphide linkages. Acorus lectins readily agglutinated rabbit, rat and guinea pig erythrocytes. Both ACL and AGL also reacted with RBCs from sheep, goat and human ABO blood groups after neuraminidase treatment. ACL and AGL were inhibited by mannose/glucose and their derivatives. The most effective inhibitor was methyl-alpha-D-mannopyranoside. Acorus lectins were stable up to 55 degrees C, did not require metal ions for their activity and were also affected by high concentrations of denaturants like urea, thiourea and guanidine-HCl. These lectins showed potent mitogenic activity towards mouse splenocytes and human lymphocytes. Both ACL and AGL also significantly inhibited the growth of J774, a murine macrophage cancer cell-line and to lesser extent WEHI-279, a B-cell lymphoma.
date: 2005-08
date_type: published
publication: International immunopharmacology
volume: 5
number: 9
publisher: Elsevier Science
pagerange: 1470-8
refereed: TRUE
issn: 1567-5769
official_url: http://www.sciencedirect.com/science/article/pii/S1567576905001025
related_url_url: http://www.sciencedirect.com/science/article/pii/S1567576905001025
related_url_type: pub
citation:   Bains, Jagmohan Singh and Dhuna, Vikram and Singh, Jatinder and Kamboj, Sukhdev Singh and Nijjar, Kamaljeet Kaur and Agrewala, J N  (2005) Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.  International immunopharmacology, 5 (9).  pp. 1470-8.  ISSN 1567-5769     
document_url: http://crdd.osdd.net/open/993/1/agrewala2005.pdf