Items where Author is "Guptasarma, Purnananda"

Sebastian Samuel, Jesse and Kumar, Deepak and Babu Chodisetti, Sathi and Agrewala, Javed N and Singh, Balvinder and Guptasarma, Purnananda and Sarkar, Dibyendu (2015) Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant. Proteins, 83 (10). pp. 1813-22. ISSN 1097-0134

Kumar, Sukhdeep and Sharma, Prerna and Arora, Kanika and Raje, Manoj and Guptasarma, Purnananda (2014) Calcium binding to beta-2-microglobulin at physiological pH drives the occurrence of conformational changes which cause the protein to precipitate into amorphous forms that subsequently transform into amyloid aggregates. PloS one, 9 (4). e95725. ISSN 1932-6203

Chandrayan, Sanjeev Kumar and Prakash, Satya and Ahmed, Shubbir and Guptasarma, Purnananda (2014) Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding. PloS one, 9 (3). e80014. ISSN 1932-6203

Prakash, Satya and Sundd, Monica and Guptasarma, Purnananda (2014) The key to the extraordinary thermal stability of P. furiosus holo-rubredoxin: iron binding-guided packing of a core aromatic cluster responsible for high kinetic stability of the native structure. PloS one, 9 (3). e89703. ISSN 1932-6203

Fatima, Uzma and Singh, Balvinder and Subramanian, Karthikeyan and Guptasarma, Purnananda (2012) Insufficient (sub-native) helix content in soluble/solid aggregates of recombinant and engineered forms of IL-2 throws light on how aggregated IL-2 is biologically active. The protein journal, 31 (7). pp. 529-43. ISSN 1875-8355

Dhaunta, Neeraj and Fatima, Uzma and Guptasarma, Purnananda (2011) N-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of α-amino groups before tryptic digestion. Analytical biochemistry, 408 (2). pp. 263-8. ISSN 1096-0309

Luthra-Guptasarma, Manni and Guptasarma, Purnananda (2010) Metal-catalyzed proteolysis, conformational antigenicity, photosensitized oxidation, and electrical dysfunction explain the pathogenicity of protein aggregates. Medical hypotheses, 75 (3). pp. 294-8. ISSN 1532-2777

Fatima, Uzma and Sharma, Swati and Guptasarma, Purnananda (2010) Structures of differently aggregated and precipitated forms of gamma B crystallin: an FTIR spectroscopic and EM study. Protein and peptide letters, 17 (9). pp. 1155-62. ISSN 1875-5305

Kapoor, Divya and Singh, Balvinder and Karthikeyan, Subramanian and Guptasarma, Purnananda (2010) A functional comparison of the TET aminopeptidases of P. furiosus and B. subtilis with a protein-engineered variant recombining the former's structure with the latter's active site. Enzyme and Microbial Technology, 46 (1). pp. 1-8. ISSN 01410229

Chandrayan, Sanjeev Kumar and Guptasarma, Purnananda (2009) Attenuation of ionic interactions profoundly lowers the kinetic thermal stability of Pyrococcus furiosus triosephosphate isomerase. Biochimica et biophysica acta, 1794 (6). pp. 905-12. ISSN 0006-3002

Kapoor, Divya and Singh, Balvinder and Subramanian, Karthikeyan and Guptasarma, Purnananda (2009) Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of betaB2 crystallin onto the hydrophobic core of gammaB crystallin. The FEBS journal, 276 (12). pp. 3341-53. ISSN 1742-4658

Kapoor, Divya and Kumar, Vijay and Chandrayan, Sanjeev Kumar and Ahmed, Shubbir and Sharma, Swati and Datt, Manish and Singh, Balvinder and Karthikeyan, Subramanian and Guptasarma, Purnananda (2008) Replacement of the active surface of a thermophile protein by that of a homologous mesophile protein through structure-guided 'protein surface grafting'. Biochimica et biophysica acta, 1784 (11). pp. 1771-6. ISSN 0006-3002

Guptasarma, Purnananda (2008) Solution-state characteristics of the ultraviolet A-induced visible fluorescence from proteins. Archives of biochemistry and biophysics, 478 (2). pp. 127-9. ISSN 1096-0384

Chandrayan, Sanjeev Kumar and Guptasarma, Purnananda (2008) Partial destabilization of native structure by a combination of heat and denaturant facilitates cold denaturation in a hyperthermophile protein. Proteins, 72 (2). pp. 539-546. ISSN 1097-0134

Sharma, Swati and Guptasarma, Purnananda (2008) Dimorphic aggregation behavior of a fusion polypeptide incorporating a stable protein domain (EGFP) with an amyloidogenic sequence (retroCspA). FEBS letters, 582 (15). pp. 2203-11. ISSN 0014-5793

Shukla, Anshuman and Raje, Manoj and Guptasarma, Purnananda (2008) Coalescence of spherical beads of retro-HSP12.6 into linear and ring-shaped amyloid nanofibers. Biochemistry. Biokhimii͡a, 73 (6). pp. 681-5. ISSN 0006-2979

Ahmed, Shubbir and Guptasarma, Purnananda (2008) Design of a soluble mini-protein through tandem duplication of the minimally engineered beta hairpin 'tongue' motif of alpha-hemolysin. Biochimie, 90 (6). pp. 957-967. ISSN 0300-9084

Chandrayan, Sanjeev Kumar and Dhaunta, Neeraj and Guptasarma, Purnananda (2008) Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures. Protein expression and purification, 59 (2). pp. 327-33. ISSN 1096-0279

Ahmed, Shubbir and Shukla, Anshuman and Guptasarma, Purnananda (2008) Folding behavior of a backbone-reversed protein: reversible polyproline type II to beta-sheet thermal transitions in retro-GroES multimers with GroES-like features. Biochimica et biophysica acta, 1784 (6). pp. 916-23. ISSN 0006-3002

Ahmed, Shubbir and Kapoor, Divya and Singh, Balvinder and Guptasarma, Purnananda (2008) Conformational behavior of polypeptides derived through simultaneous global conservative site-directed mutagenesis of chymotrypsin inhibitor 2. Biochimica et biophysica acta, 1784 (5). pp. 796-805. ISSN 0006-3002

Sharma, Swati and Guptasarma, Purnananda (2008) Evidence of native-like substructure(s) in polypeptide chains of carbonic anhydrase deposited into insoluble aggregates during thermal unfolding. The protein journal, 27 (1). pp. 50-8. ISSN 1572-3887

Verma, Archana and Sharma, Swati and Ganguly, N K and Majumdar, Siddharta and Guptasarma, Purnananda and Luthra-Guptasarma, Manni (2008) Identification and characterization of a spontaneously aggregating amyloid-forming variant of human PrP((90-231)) through phage-display screening of variants randomized between residues 101 and 112. The international journal of biochemistry & cell biology, 40 (4). pp. 663-76. ISSN 1357-2725

Shukla, Anshuman and Sharma, Swati and Guptasarma, Purnananda (2007) Confocal spectrofluorimetric evidence for the hetero-aggregation of sequence-scrambled forms of two model all-beta sheet proteins. International journal of biological macromolecules, 41 (5). pp. 650-4. ISSN 0141-8130

Kapoor, Divya and Chandrayan, Sanjeev Kumar and Ahmed, Shubbir and Guptasarma, Purnananda (2007) Using DNA sequencing electrophoresis compression artifacts as reporters of stable mRNA structures affecting gene expression. Electrophoresis, 28 (21). pp. 3862-7. ISSN 0173-0835

Bhardwaj, Sonia and Shukla, Anshuman and Mukherjee, Sourav and Sharma, Swati and Guptasarma, Purnananda and Chakraborti, Asit K and Chakrabarti, Arunaloke (2007) Putative structure and characteristics of a red water-soluble pigment secreted by Penicillium marneffei. Medical mycology : official publication of the International Society for Human and Animal Mycology, 45 (5). pp. 419-27. ISSN 1369-3786

Mukherjee, Sourav and Sharma, Swati and Kumar, Sanjeev and Guptasarma, Purnananda (2005) Slow irreversible unfolding of Pyrococcus furiosus triosephosphate isomerase: separation and quantitation of conformers through a novel electrophoretic approach. Analytical biochemistry, 347 (1). pp. 49-59. ISSN 0003-2697

Mukherjee, Sourav and Guptasarma, Purnananda (2005) Direct proteolysis-based purification of an overexpressed hyperthermophile protein from Escherichia coli lysate: a novel exploitation of the link between structural stability and proteolytic resistance. Protein expression and purification, 40 (1). pp. 71-6. ISSN 1046-5928

Shukla, Anshuman and Mukherjee, Sourav and Sharma, Swati and Agrawal, Vishal and Radha Kishan, K V and Guptasarma, Purnananda (2004) A novel UV laser-induced visible blue radiation from protein crystals and aggregates: scattering artifacts or fluorescence transitions of peptide electrons delocalized through hydrogen bonding? Archives of biochemistry and biophysics, 428 (2). pp. 144-53. ISSN 0003-9861

Kundu, Bishwajit and Shukla, Anshuman and Chaba, Rachna and Guptasarma, Purnananda (2004) The excised heat-shock domain of alphaB crystallin is a folded, proteolytically susceptible trimer with significant surface hydrophobicity and a tendency to self-aggregate upon heating. Protein expression and purification, 36 (2). pp. 263-71. ISSN 1046-5928

Shukla, Anshuman and Guptasarma, Purnananda (2004) Folding of beta/alpha-unit scrambled forms of S. cerevisiae triosephosphate isomerase: Evidence for autonomy of substructure formation and plasticity of hydrophobic and hydrogen bonding interactions in core of (beta/alpha)8-barrel. Proteins, 55 (3). pp. 548-57. ISSN 1097-0134

Shukla, Anshuman and Raje, Manoj and Guptasarma, Purnananda (2003) A backbone-reversed all-beta polypeptide (retro-CspA) folds and assembles into amyloid nanofibres. Protein engineering, 16 (12). pp. 875-9. ISSN 0269-2139

Shukla, Anshuman and Raje, Manoj and Guptasarma, Purnananda (2003) A backbone-reversed form of an all-beta alpha-crystallin domain from a small heat-shock protein (retro-HSP12.6) folds and assembles into structured multimers. The Journal of biological chemistry, 278 (29). pp. 26505-10. ISSN 0021-9258

Mukherjee, Sourav and Shukla, Anshuman and Guptasarma, Purnananda (2003) Single-step purification of a protein-folding catalyst, the SlyD peptidyl prolyl isomerase (PPI), from cytoplasmic extracts of Escherichia coli. Biotechnology and applied biochemistry, 37 (Pt 2). pp. 183-6. ISSN 0885-4513

Kundu, Bishwajit and Shukla, Anshuman and Guptasarma, Purnananda (2003) Peptide scanning-based identification of regions of gamma-II crystallin involved in thermal aggregation: evidence of the involvement of structurally analogous, helix-containing loops from the two double Greek key domains of the molecule. Archives of biochemistry and biophysics, 410 (1). pp. 69-75. ISSN 0003-9861

Maiti, Sankar and Luthra-Guptasarma, Manni and Guptasarma, Purnananda (2002) Phenomenological perspectives on the folding of beta/alpha-barrel domains through the modular formation and assembly of smaller structural elements. IUBMB life, 54 (4). pp. 213-21. ISSN 1521-6543

Kundu, Bishwajit and Guptasarma, Purnananda (2002) Use of a hydrophobic dye to indirectly probe the structural organization and conformational plasticity of molecules in amorphous aggregates of carbonic anhydrase. Biochemical and biophysical research communications, 293 (1). pp. 572-7. ISSN 0006-291X

Kundu, Bishwajit and Shukla, Anshuman and Guptasarma, Purnananda (2002) Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening. Biochemical and biophysical research communications, 291 (4). pp. 903-7. ISSN 0006-291X

Kundu, Bishwajit and Guptasarma, Purnananda (1999) Hydrophobic dye inhibits aggregation of molten carbonic anhydrase during thermal unfolding and refolding. Proteins, 37 (3). pp. 321-4. ISSN 0887-3585

Guptasarma, Purnananda (1999) Proposing T-independent B-cell activation by prion rods. Could disease result from 'chaperoning' of nascent prions by PrPsc-cognate immunoglobulins? Current Science, 77 (4). pp. 508-514.

Guptasarma, Purnananda (1997) Resolving multiple protein conformers in equilibrium unfolding reactions: a time-resolved emission spectroscopic (TRES) study of Azurin. Biophysical chemistry, 65 (2-3). pp. 221-8. ISSN 0301-4622