1AOD	Hydrolase	date	Jul 02, 1997
title	Phosphatidylinositol-Specific Phospholipase C From Listeria Monocytogenes
authors	D.W.Heinz, J.Moser
compound		source
Molecule: Phosphatidylinositol-Specific Phospholipase C Chain: A Synonym: Pi-Plc Ec: 3.1.4.10 Engineered: Yes Other_details: Bound Myo-Inositol In Active Site		Organism_scientific: Listeria Monocytogenes Strain: Egd Serotype 12a Cell_line: Bl21 Cellular_location: Secreted Into Medium Gene: Plca Expression_system: Escherichia Coli Expression_system_strain: Bl21 (De3) Expression_system_cellular_location: Cytoplasm Expression_system_vector_type: Plasmid Expression_system_plasmid: Plca Expression_system_gene: Plca Deletion Variant
symmetry	Space Group: P 31 2 1	R_factor	0.158
crystal cell	length a length b length c angle alpha angle beta angle gamma 82.100 82.100 92.100 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.60 Å
ligand	INS	enzyme	Lyase E.C.4.6.1.13 BRENDA
related structures	by homologous chain: 2PLC
similarity	Contains 1 pi-plc x-box domain.[PI-PLC-X]
subunit	Monomer.
catalytic activ.	1-phosphatidyl-1d-myo-inositol = 1d-myo- inositol 1,2-cyclic phosphate + diacylglycerol.
subcellular loc.	Membrane localization by OPM: Secreted out side in side
Genes	LMO0201, PIC, PLCA (L. monocytogenes)
function	Important factor in pathogenesis, pi-plc activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane. Cleaves glycosylphosphatidylinositol (gpi) and phosphatidylinositol (pi) anchors but not pi phosphates.
Gene Ontology	Chain Function Process Component A phosphatidylinositol diacylg... phospholipase C activity lyase activity signal transduction intracellular signaling casc... pathogenesis lipid catabolic process cytoplasm
Primary reference	Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes., Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW, J Mol Biol 1997 Oct 17;273(1):269-82. PMID:9367761

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (51 Kb) [Save to disk] Biological Unit Coordinates (1aod.pdb1.gz) 47 Kb CSU: Contacts of Structural Units for 1AOD Likely Quarternary Molecular Structure file(s) for 1AOD Structure Factors (257 Kb) Retrieve 1AOD in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1AOD, from MSDmotif at EBI Genome occurence of 1AOD's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1aod__, region [Jmol] [rasmolscript] [script source] Fold representative 1aod from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1aod_A]

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