1FJ1	Immune System	date	Aug 07, 2000
title	Lyme Disease Antigen Ospa In Complex With Neutralizing Antibody Fab La-2
authors	W.Ding, C.L.Lawson
compound		source
Molecule: Hybridoma Antibody La2 (Light Chain) Chain: A, C Fragment: Fab Fragment Synonym: La2 Fab		Organism_scientific: Mus Musculus Organism_common: Mouse Other_details: La-2 Hybridoma, Gift Of M.M. Simon, Max Planck Instutut, Freiburg;
Molecule: Hybridoma Antibody La2 (Heavy Chain) Chain: B, D Fragment: Fab Fragment Synonym: La2 Fab		Organism_scientific: Mus Musculus Organism_common: Mouse Other_details: La-2 Hybridoma
Molecule: Outer Surface Protein A Chain: E, F Synonym: Ospa Engineered: Yes		Organism_scientific: Borrelia Burgdorferi Organism_common: Lyme Disease Spirochete Strain: B31 Expression_system: Escherichia Coli Expression_system_plasmid: Pet9c
symmetry	Space Group: P 21 21 2	R_factor	0.226
crystal cell	length a length b length c angle alpha angle beta angle gamma 100.079 129.462 143.738 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.68 Å
note	1FJ1 supersedes 2OSP
related structures	by homologous chain: 1CBV, 1CF8, 1FVE, 1RJL
similarity	Belongs to the Lipoprotein_1 family.
subcellular loc.	Attached to the outer membrane by a lipid anchor.
Gene	BB ; OSPA (B. burgdorferi)
Gene Ontology	Chain Function Process Component F, E cell outer membrane membrane outer membrane
Primary reference	Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA., Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL, J Mol Biol 2000 Oct 6;302(5):1153-64. PMID:11183781

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (202 Kb) [Save to disk] Biological Unit Coordinates (1fj1.pdb1.gz) 104 Kb Biological Unit Coordinates (1fj1.pdb2.gz) 98 Kb CSU: Contacts of Structural Units for 1FJ1 Likely Quarternary Molecular Structure file(s) for 1FJ1 Structure Factors (394 Kb) Retrieve 1FJ1 in mmCIF format [Save to disk]

View 1FJ1 in 3D

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Visual 3D analysis of 1FJ1

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1FJ1, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1fj1a1, region A:1-107 [Jmol] [rasmolscript] [script source]     - Domain d1fj1a2, region A:108-213 [Jmol] [rasmolscript] [script source]     - Domain d1fj1b1, region B:1-114 [Jmol] [rasmolscript] [script source]     - Domain d1fj1b2, region B:115-213 [Jmol] [rasmolscript] [script source]     - Domain d1fj1c1, region C:1-107 [Jmol] [rasmolscript] [script source]     - Domain d1fj1c2, region C:108-213 [Jmol] [rasmolscript] [script source]     - Domain d1fj1d1, region D:1-114 [Jmol] [rasmolscript] [script source]     - Domain d1fj1d2, region D:115-213 [Jmol] [rasmolscript] [script source]     - Domain d1fj1e_, region E [Jmol] [rasmolscript] [script source]     - Domain d1fj1f_, region F [Jmol] [rasmolscript] [script source] Fold representative 1fj1 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1fj1_D] [1fj1_C] [1fj1_B] [1fj1_A] [1fj1_E] [1fj1_F]

Other resources with information on 1FJ1

InterPro: IPR001809 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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