1HY5	Toxin	date	Jan 18, 2001
title	Crystal Structure Of The Catalytic Domain Of Yope-Yersinia Pestis Gap Effector Protein.
authors	A.G.Evdokimov, J.E.Tropea, K.M.Routzahn, D.S.Waugh
compound		source
Molecule: Yersinia Pestis Virulence Protein Yope Chain: A, B Fragment: C-Terminal Domain (Residues 90-219); Bacterial Gtpase Activating Protein (Gap Domain); Synonym: Yope, Yersinia Outer Protein E Engineered: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Yope Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(Ril)De3 Expression_system_vector_type: Plasmid
symmetry	Space Group: C 1 2 1	R_factor
crystal cell	length a length b length c angle alpha angle beta angle gamma 62.692 71.978 62.684 90.00 113.06 90.00
method	X-Ray Diffraction	resolution	2.25 Å
ligand	MSE	enzyme
note	1HY5 is a representative structure
similarity	Belongs to the YopE family.
induction	At 37 degrees celsius in the absence of calcium.
subcellular loc.	Outer membrane.
Genes	Y0077, Y5068, YOP25, YOPE, YPCD1.06 (Y. pestis); YP (Y. pestis)
function	Essential virulence determinant; cytotoxic effector, involved in resistance to phagocytosis.
Gene Ontology	Chain Function Process Component A, B GTPase activator activity pathogenesis membrane outer membrane
Primary reference	Crystal structure of the Yersinia pestis GTPase activator YopE., Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS, Protein Sci 2002 Feb;11(2):401-8. PMID:11790850

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (42 Kb) [Save to disk] Biological Unit Coordinates (1hy5.pdb1.gz) 21 Kb Biological Unit Coordinates (1hy5.pdb2.gz) 19 Kb CSU: Contacts of Structural Units for 1HY5 Likely Quarternary Molecular Structure file(s) for 1HY5 Structure Factors (292 Kb) Retrieve 1HY5 in mmCIF format [Save to disk]

View 1HY5 in 3D

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1HY5, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1hy5a_, region A [Jmol] [rasmolscript] [script source]     - Domain d1hy5b_, region B [Jmol] [rasmolscript] [script source] Fold representative 1hy5 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1hy5_A] [1hy5_B]

Other resources with information on 1HY5

InterPro: IPR003537 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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