1PFO	Toxin	date	Jul 31, 1997
title	Perfringolysin O
authors	J.Rossjohn, M.W.Parker
compound		source
Molecule: Perfringolysin O Chain: A Synonym: Pfo Engineered: Yes		Organism_scientific: Clostridium Perfringens Cellular_location: Cytoplasm Expression_system: Escherichia Coli Other_details: Secreted As A Monomer, Forms Membrane-Bound Oligomers
symmetry	Space Group: C 2 2 21	R_factor	0.211
crystal cell	length a length b length c angle alpha angle beta angle gamma 46.987 178.292 174.207 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.20 Å
note	1PFO is a representative structure
related structures	by homologous chain: 1M3I
similarity	Belongs to the thiol-activated cytolysin family.[Thiol_cytolysin]
subunit	Forms oligomers in the host membrane.
subcellular loc.	Membrane localization by OPM: Secreted out side in side
Genes	CPE0163, PFOA, PFOR, PFO (C. perfringens)
function	Cholesterol is the receptor for the binding of these toxins to eukaryotic cell membranes. Is able to lyse cholesterol containing membranes. Sulfhydryl-activated toxin. Can be reversibly inactivated by oxidation.
Gene Ontology	Chain Function Process Component A lipid binding cholesterol binding pathogenesis cytolysis hemolysis by symbiont of hos...
Primary reference	Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form., Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW, Cell 1997 May 30;89(5):685-92. PMID:9182756

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (87 Kb) [Save to disk] Biological Unit Coordinates (1pfo.pdb1.gz) 156 Kb CSU: Contacts of Structural Units for 1PFO Likely Quarternary Molecular Structure file(s) for 1PFO Retrieve 1PFO in mmCIF format [Save to disk]

View 1PFO in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1PFO

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1PFO, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1pfo__, region [Jmol] [rasmolscript] [script source] Fold representative 1pfo from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1pfo_A]

Other resources with information on 1PFO

InterPro: IPR001869 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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