1SJ2	Oxidoreductase	date	Mar 02, 2004
title	Crystal Structure Of Mycobacterium Tuberculosis Catalase- Peroxidase
authors	T.Bertrand, N.A.J.Eady, J.N.Jones, J.Bodiguel, Jesmin, J.M.Nagy, E.L.Raven, B.Jamart-Gregoire, K.A.Brown
compound		source
Molecule: Peroxidasecatalase T Chain: A, B Synonym: Catalase-Peroxidase T Ec: 1.11.1.6 Engineered: Yes		Organism_scientific: Mycobacterium Tuberculosis Gene: Katg, Rv1908c, Mt1959, Mtcy180.10 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Um255 Expression_system_vector_type: Plasmid Expression_system_plasmid: Ptrc99a
symmetry	Space Group: P 42 21 2	R_factor	0.211
crystal cell	length a length b length c angle alpha angle beta angle gamma 150.330 150.330 154.281 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.41 Å
ligand	GOL, HEM	enzyme	Catalase;. Equilase;. Caperase;. Optidase;. Catalase-peroxidase;. CAT. Oxidoreductase E.C.1.11.1.6 BRENDA
similarity	Bacterial peroxidase/catalase subfamily. Belongs to the peroxidase family.
subunit	Homodimer (probable).
catalytic activ.	2 h(2)o(2) = o(2) + 2 h(2)o.
genes	CAT (B. taurus); katE, katG (E. coli); perA (H. marismortui); katA (H. pylori); CAT (H. sapiens); katA (M. luteus); MT1959, katG (M. tuberculosis); cat-1 (N. crassa); katA (P. mirabilis); katB (P. syringae); CTA1 (S. cerevisiae)
function	May play a role in the intracellular survival of mycobacteria. Bifunctional, exhibiting both a catalase and broad- spectrum peroxidase activities.
Gene Ontology	Chain Function Process Component A, B catalase activity peroxidase activity iron ion binding oxidoreductase activity metal ion binding electron transport response to oxidative stress... hydrogen peroxide catabolic ...
disease	Acatalasemia Glycine N-Acyltransferase; Glyat
Primary reference	Crystal structure of Mycobacterium tuberculosis catalase-peroxidase., Bertrand T, Eady NA, Jones JN, Nagy JM, Jamart-Gregoire B, Raven EL, Brown KA, J Biol Chem 2004 Jul 1;. PMID:15231843

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (262 Kb) [Save to disk] Biological Unit Coordinates (1sj2.pdb1.gz) 239 Kb CSU: Contacts of Structural Units for 1SJ2 Likely Quarternary Molecular Structure file(s) for 1SJ2 Structure Factors (514 Kb) Retrieve 1SJ2 in mmCIF format [Save to disk]

View 1SJ2 in 3D

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Visual 3D analysis of 1SJ2

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1SJ2, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1sj2a1, region A:24-435 [Jmol] [rasmolscript] [script source]     - Domain d1sj2a2, region A:436-720 [Jmol] [rasmolscript] [script source]     - Domain d1sj2b1, region B:24-435 [Jmol] [rasmolscript] [script source]     - Domain d1sj2b2, region B:436-720 [Jmol] [rasmolscript] [script source] Fold representative 1sj2 from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1sj2_B] [1sj2_A]

Other resources with information on 1SJ2

InterPro: IPR000763 , IPR002016 MMDB (Entrez's Structure Database)

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