Epidermal Growth Factor Receptor Inhibitor Database
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| Experimental Information | |
| Enzymatic Activity | |
| Molecule Id | EGIN0000003 |
| PMID | 22372864 |
| Assay Used | Kinase and Cellular assay |
| Experimently Validated | Yes |
| EGFR (IC50) | 52.2 nM |
| HER2 (IC50) | 36.3 nM |
| HER4 (IC50) | > 1000 nM |
| Mutant L858R (IC50) | - |
| Mutant T790M (IC50) | 1000 nM |
| Double Mutant (IC50) | - |
| Average ERBB2 and ERBB1 (IC50) | - |
| Inhibition EGFR (%) | - |
| Inhibitory Concentration | - |
| Inhibition HER2 (%) | - |
| Inhibitory Concentration | - |
| Ki (EGFR) | - |
| Ki (HER2) | - |
| Ki (HER4) | - |
| Kd (EGFR) | - |
| Kd (L858R) | - |
| Kd (T790M) | - |
| Kd (L858R/T790M) | - |
| Kd (Other mutation) | - |
| Note | The paper describes the Synthesis and Biological Evaluation of Pyrimidine-Based Dual Inhibitors of Human Epidermal Growth Factor Receptor 1 (HER-1) and HER-2 Tyrosine Kinases |
| Extra | - |
| Cellular Activity | ||||||||||||||
| S.No. | Cell Line | Desp. | IC50 | IC80 | ED50 | GI50 | GI90 | EC50 | Kd | Inhibition (%) | Inhibitory Conc. | |||
| 1 | A431 | - | 104 nM | - | - | - | - | - | - | - | - | |||
| 2 | H1781 | HER-2 G776insV_G/C | 3047 nM | - | - | - | - | - | - | - | - | |||
| 3 | H1975 | EGFR L858R/T790M | > 1000 nM | - | - | - | - | - | - | - | - | |||
| 4 | JIMT-1 | low HER-2 | > 10000 nM | - | - | - | - | - | - | - | - | |||
| 5 | MDA-MB-175 | Src++, Ret+++, low HER-2 | 44.4 nM | - | - | - | - | - | - | - | - | |||
| 6 | MDA-MB-361 | Her-2++, Src+++, PIK3CA mutation, ER activation | 1029 nM | - | - | - | - | - | - | - | - | |||
| 7 | MDA-MB-453 | HER-2++, PTEN deficiency, PIK3CA mutation | 555 nM | - | - | - | - | - | - | - | - | |||
| 8 | N87 | HER-2+++ | 36 nM | - | - | - | - | - | - | - | - | |||
| 9 | SKBR3 | - | 29 nM | - | - | - | - | - | - | - | - | |||
| Activity against Receptor | |
| EGFR | Yes |
| HER2 | Yes |
| HER4 | Yes |
| Wild type | Yes |
| Mutant | T790M |
| Inhibitor Binding | |
| EGFR | CYS773 |
| HER2 | CYS805 |
| HER4 | - |