Guanosine-5'-triphosphate (GTP) is a purine nucleotide.GTP is involved in energy transfer or an activator of substrates in biochemical reactions. GTP Binding proteins are regulatory proteins which becomes activated when bound to GTP and is involved in signal transduction. They including the G proteins and the monomeric small GTP-binding proteins, in which the exchange of GDP for GTP induces a conformational change to produce an active state, while the hydrolysis of GTP to GDP produces an inactive state. GTP-binding proteins act as switches that couple cell-surface receptor activation with intracellular processes such as protein synthesis.

GTP Binder

GTPBinder: is a web based tool for prediction of GTP binding residue in protein sequence.It takes amino acid sequence in FASTA format as input and predict the GTP-interacting residues with Support Vector Machine (SVM). These method have been trained and tested using five-fold cross-validation. Residue-wise detail prediction by GTPBinder is given in tabular format. This table contain three columns (i) amino acid residue, (ii) SVM score and (iii) prediction class.

Please cite following reference if you are using GTPbinder
Chauhan, J. S., Mishra, N. K. and Raghava, G. P. S. (2010) Prediction of GTP interacting residues, dipeptides and tripeptides in a protein from its evolutionary information BMC Bioinformatics 2010, 11:301.

© Raghava's group, 2009.Bioinformatics Centre
Institute of Microbial Technology, Chandigarh, India