PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 41

Entry No. - 1   [TOP]
PolysacDB ID1114
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 5b   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure108568
Proposed functionsLPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemElisa inhibition assay
Cross-reactivityThis antibody reacted only with the reference strains of Actinobacillus serotypes 5
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used for identification of serotype5 cells
Curator IDAA + AS
Date of Curation02-02-2010
ReferencesPMC1189361


Entry No. - 2   [TOP]
PolysacDB ID1613
Carbohydrate NamePTP1 antigen   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeEncephalitozoon intestinalis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesInfected patient
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemIndirect immunofluorescence, Western blotting, two-dimensional gel electrophoresis, and chemical deglycosylation
Cross-reactivityThis antisera cross-reacted with E. hellem and E. cuniculi
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera was found to decrease the infectivity of E. intestinalis in vitro
Curator IDAA + AS
Date of Curation23-09-2010
ReferencesPMC1307029


Entry No. - 3   [TOP]
PolysacDB ID1903
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePasteurella multocida serotype 1   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides. The O-antigen part consists of the following residues : -3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp-(1-. The core oligosaccharide part consists of the following residues : β-D-Galp-(1-->7)-D-gro-α-D-manHepp-(1-->6)-D-gro-α-D-manHepp-(1-->6)-β-D-Glcp-(1-->4)-L-gro [branched to L-gro-α-D-manHepp-(1-->2)-L-gro-α-D-manHepp-(1-->3)] and [branched to α-D-Glcp-(1-->6)] -α-D-manHepp-(1--/lipid A
BCSDB StructureN/A
Proposed functionsLPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antisera was specific to serotype 1 cells
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera may be useful in defining antigenic determinants of LPS.
Curator IDAA + AS
Date of Curation25-11-2010
ReferencesPMC271284


Entry No. - 4   [TOP]
PolysacDB ID1904
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePasteurella multocida serotype 15   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB StructureN/A
Proposed functionsLPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antisera was specific to serotype 15 cells
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera may be useful in defining antigenic determinants of LPS.
Curator IDAA + AS
Date of Curation25-11-2010
ReferencesPMC271284


Entry No. - 5   [TOP]
PolysacDB ID1905
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePasteurella multocida serotype 4   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB StructureN/A
Proposed functionsLPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antisera cross-reacted with serotype 12 cells
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera may be useful in defining antigenic determinants of LPS.
Curator IDAA + AS
Date of Curation25-11-2010
ReferencesPMC271284


Entry No. - 6   [TOP]
PolysacDB ID1906
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePasteurella multocida serotype 12   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB StructureN/A
Proposed functionsLPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antisera cross-reacted with serotype 4 cells
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera may be useful in defining antigenic determinants of LPS.
Curator IDAA + AS
Date of Curation25-11-2010
ReferencesPMC271284


Entry No. - 7   [TOP]
PolysacDB ID1999
Carbohydrate NameDisaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the disaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-[5-->P-->(CH2)3NH2]
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


Entry No. - 8   [TOP]
PolysacDB ID2000
Carbohydrate NameTrisaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the trisaccharide is -D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


Entry No. - 9   [TOP]
PolysacDB ID2001
Carbohydrate NameTetrasaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera cross-reacted with capsular polysaccharide of 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B and 6A challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


Entry No. - 10   [TOP]
PolysacDB ID2071
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa strain Fisher IT-3   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAlkali treated LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivityThis antisera was highly cross-reactive
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera had opsonophagocytic activity against LPS rough strains
Curator IDAA + AS
Date of Curation07-01-2011
ReferencesPMC172952


Entry No. - 11   [TOP]
PolysacDB ID2072
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa strain Fisher IT-4   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAlkali treated LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivityThis antisera was specific to strain IT-4
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera had opsonophagocytic activity against LPS rough strains
Curator IDAA + AS
Date of Curation07-01-2011
ReferencesPMC172952


Entry No. - 12   [TOP]
PolysacDB ID2073
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa strain AK1401   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB Structure4396
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAlkali treated LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivityThis antisera was highly cross-reactive
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera had opsonophagocytic activity
Curator IDAA + AS
Date of Curation08-01-2011
ReferencesPMC172952


Entry No. - 13   [TOP]
PolysacDB ID2270
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityThe polyclonal rabbit anti-group antigen 3.4 antibodies reacted with the lb, 2a, 3b, 4a. 4b, and Y LPS. They did not bind to1a and 5a. In glycoconjugate ELISA's it bound to all saccharides especially abcd- and decasaccharide- BSA antigens
Proposed epitopesAntigen 3,4 seemed to be the epitope
IEDB Epitope76696
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation16-03-2011
References2438343


Entry No. - 14   [TOP]
PolysacDB ID2323
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThe highest titers seen were against serotype 3a and 3b LPSs
Proposed epitopesIn S. flexneri serotype 3a and 3b LPSs there is no structural feature to which the type III antigen epitope can be assigned. Instead, the serotype specificity is a consequence of the addition of two group antigen epitopes to the basic structure in serotype 3a (7,8 and 6 epitopes) and of one group antigen epitope to the basic structure in serotype 3b (6 epitope). The α-D-glucose-(1-->3)-α-L-rhamnose was assigned as the determinant for group 7,8 antigen, and the 2-O-acetyl-α-L-rhamnose III structure was assigned as the determinant for group 6 antigen
IEDB Epitope76815
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation02-04-2011
ReferencesPMC96386


Entry No. - 15   [TOP]
PolysacDB ID2324
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)]   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB StructureN/A
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgG
Assay SystemELISA
Cross-reactivityThis antisera strongly cross-reacted with S. flexneri LPS of serotypes 1a and 1b
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation02-04-2011
ReferencesPMC96386


Entry No. - 16   [TOP]
PolysacDB ID2325
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)]   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB StructureN/A
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgA
Assay SystemELISA
Cross-reactivityThis antisera showed an extensive cross-reaction that included S. flexneri serotypes 1a, 1b, 2b, 4b, 5b, and Y
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityCross-protection was observed among S. flexneri serotypes elicited by the S. flexneri 2a/3a vaccine
Curator IDAA + AS
Date of Curation02-04-2011
ReferencesPMC96386


Entry No. - 17   [TOP]
PolysacDB ID2353
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSinorhizobium meliloti strain RM41   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThere are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB StructureN/A
Proposed functionsLPS is an important surface antigen
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA and immunoblotting
Cross-reactivityThis antisera was specific to the homologous immunizing strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species
Curator IDAA + AS
Date of Curation13-04-2011
References10543844


Entry No. - 18   [TOP]
PolysacDB ID2354
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSinorhizobium fredii USDA205   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThere are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB StructureN/A
Proposed functionsLPS is an important surface antigen
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA and immunoblotting
Cross-reactivityThis antisera was specific to the homologous immunizing strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species
Curator IDAA + AS
Date of Curation13-04-2011
References10543844


Entry No. - 19   [TOP]
PolysacDB ID2386
Carbohydrate NameStreptococcal polysaccharide antigen eI   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus mutans strain MT703 serotype e   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe major fraction (eI) was identified as a polysaccharide composed of 37% glucose, 56% rhamnose, 5% protein, and 0.3% phosphorus, whereas the minor fraction (eII) contained 66% protein in addition to 10% glucose and 17% rhamnose
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemQuantitative precipitin test and immunodiffusion and/or immunoelectrophoresis
Cross-reactivityThis antisera cross-reacted with type E
Proposed epitopesMethyl-β-D-glucopyranoside residues seemed to be the predominant epitope. A β-linked glucose-glucose dimer is the predominant antigenic determinant of the e specificity
IEDB EpitopeN/A
Proposed UtilityThis antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans
Curator IDAA + AS
Date of Curation25-04-2011
ReferencesPMC420845


Entry No. - 20   [TOP]
PolysacDB ID2387
Carbohydrate NameStreptococcal polysaccharide antigen eI   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus mutans strain K129 serotype E   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemQuantitative precipitin test and immunodiffusion and/or immunoelectrophoresis
Cross-reactivityThis antisera was specific to E1
Proposed epitopesMethyl-β-D-glucopyranoside residues seemed to be the predominant epitope
IEDB EpitopeN/A
Proposed UtilityThis antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans
Curator IDAA + AS
Date of Curation25-04-2011
ReferencesPMC420845


Entry No. - 21   [TOP]
PolysacDB ID2388
Carbohydrate NameCell wall carbohydrate   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus GAS strain J-17   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesInfected patients
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgG2
Assay SystemELISA
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae
Curator IDAA + AS
Date of Curation25-04-2011
References8810949


Entry No. - 22   [TOP]
PolysacDB ID2389
Carbohydrate NameCell wall carbohydrate   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus GAS strain J-18   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgG2
Assay SystemELISA
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae
Curator IDAA + AS
Date of Curation26-04-2011
References8810949


Entry No. - 23   [TOP]
PolysacDB ID2390
Carbohydrate NameType III polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus Group B strain M731 and M732   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis polysaccharide has a mol wt of > 5 × 106 daltons. Its main chemical constituents are sialic acid (24%), galactose (25%), heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%)
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemCapillary precipitin tests, immunodiffusion test
Cross-reactivityThis antisera predominantly reacted with type III polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation26-04-2011
References55450


Entry No. - 24   [TOP]
PolysacDB ID2409
Carbohydrate NameGroup B antigen   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus Group B strain M732   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis polysaccharide had a molecular size > 5 x 106. Its main chemical constituents were sialic acid (24%), galactose (25%), a sugar tentatively identified as heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%)
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin-killed whole cell vaccines
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemCapillary precipitin tests, immunodiffusion tests, immunoelectrophoresis
Cross-reactivityThis antisera cross-reacted with the TCA antigenas well as the EDTA-non pH titrated antigen and the group B antigen extracted with formamide from strain 090R or D136C
Proposed epitopesL-rhamnose was the immunodominant sugar for the formamide-extracted B antigen
IEDB EpitopeN/A
Proposed UtilityThis antisera has helped determine that two antigens that react identically with each other and with similar specificities of antibody, yet with different immunodominant determinants
Curator IDAA + AS
Date of Curation02-05-2011
References80423


Entry No. - 25   [TOP]
PolysacDB ID2412
Carbohydrate NamePhosphorylcholine (PC)   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsAntibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
Antigenic Nature used to produce antibodiesASRNKANDYTTEYSASVKGRFIVS [peptide1]
Carrier NameBovine serum albumin
Conjugation MethodBSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, Opsonization and protection assays
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
Curator IDAA + AS
Date of Curation03-05-2011
References10992485


Entry No. - 26   [TOP]
PolysacDB ID2413
Carbohydrate NameType V capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus Group B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe native type v polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-glucose and sialic acid in a molar ratio of 3:2:1:1. It consists of a repeating backbone of -->4)-α-D-Glcp-(1-->4)-α-D-Galp-(1-->4)-β-D-Glcp-(1--> with branching at 6th and 3rd positions
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, Ouchterlony diffusion
Cross-reactivityAntibodies against the typeV capsuled o not cross-reactw ith other GBS capsular types and appear to recognize an epitope which is not as heavily dependent on the presenceo f the terminal side chain sialic acid residues as that of the type Ia, 11, and I11 GBS polysaccharides
Proposed epitopesβ-D-Gal-(1-->4)-β-D-GlcNAc segmenot of the trisaccharide side chain, which is present in the core but not in the backbone polysaccharide, also contributes to the native epitope. The residues of the linear backbone and/or the glucopyranosyl side chain also form part of the antibody-binding epitope
IEDB EpitopeN/A
Proposed UtilityThis antisera helped in the determination of immunodominant epitopes in Type V polysaccharide in group B Streptococcus
Curator IDAA + AS
Date of Curation04-05-2011
References2016287


Entry No. - 27   [TOP]
PolysacDB ID2421
Carbohydrate NameType 1 polysaccharide antigen   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus sanguis ST3 Serotype I   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe type I antigen was a polysaccharide composed of glucose, rhamnose, and N-acetylglucosamine in a molar ratio of 1.4:2.5:1.0.
BCSDB StructureN/A
Proposed functionsThe serotype I antigen may participate in the attachment of S. sanguis to tooth surfaces
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemQuantitative precipitin inhibition tests, Immunodiffusion and immunoelectrophoresis
Cross-reactivityN/A
Proposed epitopesα-glucosidic linkage is the immunodeterminant of the type I antigen
IEDB EpitopeN/A
Proposed UtilityThis antisera helped in determining the immunodominant region in the Type 1 polysaccharide
Curator IDAA + AS
Date of Curation07-05-2011
ReferencesPMC347986


Entry No. - 28   [TOP]
PolysacDB ID2422
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus Group B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site
BCSDB StructureN/A
Proposed functionsThe property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodType III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C. Adipic acid dihydrazide was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The CP-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and TT per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column
AntibodiesAntisera
Antibody type and classIgG
Assay SystemDouble immunodiffusion and capillary precipitation, opsonization assays
Cross-reactivityThis antisera was quite specific and did not react with the structurally related pneumococcus type 14 polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityConjugate-induced antibodies facilitated opsonization of group B streptococcus type III organisms. The type III-TT conjugate can be a potential vaccine for prevention of neonatal Group B Streptococcal diseases.
Curator IDAA + AS
Date of Curation08-05-2011
References2407652


Entry No. - 29   [TOP]
PolysacDB ID2426
Carbohydrate NameStreptococcal A carbohydrate   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus Group A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsThis Carbohydrate is highly immunodominant
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemRadioimmunoassay
Cross-reactivityN/A
Proposed epitopesThe antigenic determinant was known as α hapten that had a estimated molecular weight of 1100 daltons and a rhamnose: glucosamine molar ratio of 6:1
IEDB Epitope76687
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation10-05-2011
References4795261


Entry No. - 30   [TOP]
PolysacDB ID2435
Carbohydrate NameTrisaccharide part of Streptococcus pneumoniae type 3 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae Type 3   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodThe saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5
AntibodiesAntisera
Antibody type and classN/A
Assay SystemEnzyme-linked immunosorbent assay and in-vitro phagocytosis assay
Cross-reactivityN/A
Proposed epitopesβ-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp
IEDB EpitopeN/A
Proposed UtilityThis antisera provided full protection against challenge with pneumococcal type 3 bacteria
Curator IDAA + AS
Date of Curation13-05-2011
ReferencesPMC308892


Entry No. - 31   [TOP]
PolysacDB ID2461
Carbohydrate NameSynthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureSynthetic disaccharide abequose1-->3α-rhamnose
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugate
Carrier NameBovine serum albumin
Conjugation MethodN/A
AntibodiesAntisera
Antibody type and classN/A
Assay SystemImmunofluorescence and co-agglutination (COA)
Cross-reactivityThe antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci
Curator IDAA + AS
Date of Curation23-05-2011
References373381


Entry No. - 32   [TOP]
PolysacDB ID2463
Carbohydrate NamePolysaccharide antigen (PS 02/725)   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEubacterium saburreum strain 02/725   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis polysaccharide consists of D-glycero-d-galacto-heptose and 6-deoxyheptose residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgG
Assay SystemPrecipitin assay and complement binding assay
Cross-reactivityThis antisera was specific to 02/725 polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation24-05-2011
References


Entry No. - 33   [TOP]
PolysacDB ID2464
Carbohydrate NameSurface polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEubacterium saburreum strain T18   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe purified antigen was a homopolysaccharide composed of D-glycero-D-galacto-heptose (Hep.) residues. The structure of the repeating unit in the polysaccharide was: -[-6)-[α Hep.furanosyl-(1-->4)]-β-Hep.pyranosyl- (1-->6)-[α-Hep.furanosyl-(1-->2), α-Hep.furanosyl-(1-->4)]-β- Hep.pyranosyl-(1-)4-->6)-β-Hep.pyranosyl-(1-->. No heptose residues were acetylated
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemImmunodiffusion tests
Cross-reactivityN/A
Proposed epitopesThe immunodeterminant of the antigenic polysaccharide was D-glycero-D-galacto-heptofuranosyl residues as branched nonreducing terminals
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation25-05-2011
References1408355


Entry No. - 34   [TOP]
PolysacDB ID2465
Carbohydrate NamePolysaccharide antigen PS L13   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEubacterium saburreum L13   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis polysaccharide contained ketohexose and glucose residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgG
Assay SystemDouble diffusion experiments, Immunoelectrophoresis
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityRabbit antiserum to E. saburreum L13 agglutinated the bacteria
Curator IDAA + AS
Date of Curation25-05-2011
ReferencesPMC347922


Entry No. - 35   [TOP]
PolysacDB ID2469
Carbohydrate NameSurface polysaccharide antigen   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEubacterium saburreum strain L32   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe PS L32 polymer is built up of repeating trisaccharide units of galactose, ribose and dideoxyhexose
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgM
Assay SystemPrecipitation tests, immunoelectrophoresis and complement fixation
Cross-reactivityN/A
Proposed epitopesThe dideoxyhexose is the immunodominant sugar
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation27-05-2011
References670929


Entry No. - 36   [TOP]
PolysacDB ID2470
Carbohydrate NameSurface polysaccharide antigen   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEubacterium saburreum strain T18   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis antigen was composed of D-glycero-D-galacto-heptose
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classIgM
Assay SystemImmunoprecipitation, immunodiffusion
Cross-reactivityThis antisera was specific to T18 antigen and did not cross-react with L44 antigen and T27 antigen
Proposed epitopesD-glycero-D-galacto-heptose constitutes part of the immunodeterminant of the antigenic polysaccharide
IEDB EpitopeN/A
Proposed UtilityThe polysaccharide antigen from strain T18 is distinct from those previously reported for this species and may be useful in serotyping E. saburreum
Curator IDAA + AS
Date of Curation28-05-2011
ReferencesPMC260431


Entry No. - 37   [TOP]
PolysacDB ID2495
Carbohydrate NameO-polysaccharide [Terminal hexasaccharides]   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae O1 LPS serotype ogawa   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar is characterized by a 2-O-methyl group
BCSDB Structure6642
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
AntibodiesAntisera
Antibody type and classIgG and IgM
Assay SystemELISA
Cross-reactivityN/A
Proposed epitopesThe epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
IEDB Epitope76824
Proposed UtilityThis antisera showed protection
Curator IDAA + AS
Date of Curation04-06-2011
References11920320


Entry No. - 38   [TOP]
PolysacDB ID2496
Carbohydrate NameO-polysaccharide [Terminal disaccharide]   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae O1 LPS serotype Inaba   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure6642
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
AntibodiesAntisera
Antibody type and classIgM and IgG1
Assay SystemELISA
Cross-reactivityThis antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopesN/A
IEDB Epitope76775
Proposed UtilityThis antisera was neither vibriocidal nor protective in the infant mouse cholera model
Curator IDAA + AS
Date of Curation05-06-2011
ReferencesPMC427411


Entry No. - 39   [TOP]
PolysacDB ID2497
Carbohydrate NameO-polysaccharide [Terminal trisaccharide]   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae O1 LPS serotype Inaba   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure6642
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
AntibodiesAntisera
Antibody type and classIgM and IgG2
Assay SystemELISA
Cross-reactivityThis antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopesN/A
IEDB Epitope76775
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation05-06-2011
ReferencesPMC427411


Entry No. - 40   [TOP]
PolysacDB ID2498
Carbohydrate NameO-polysaccharide [Terminal tetrasaccharide]   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae O1 LPS serotype Inaba   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure6642
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
AntibodiesAntisera
Antibody type and classIgM and IgG3
Assay SystemELISA, invivo protection assay
Cross-reactivityThis antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopesN/A
IEDB Epitope76775
Proposed UtilityThis antisera helped in the epitope studies of O1 LPS
Curator IDAA + AS
Date of Curation05-06-2011
ReferencesPMC427411


Entry No. - 41   [TOP]
PolysacDB ID2502
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae Non-O1 serogroup   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cell bacteria and purified LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antisera was specific to V. cholerae had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera can be used for the detection of Vibrio cholerae non O1 strrains
Curator IDAA + AS
Date of Curation08-06-2011
ReferencesTUMS Journals


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036