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PolysacDB ID | 1114 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 5b (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
BCSDB Structure | 108568 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Elisa inhibition assay |
Cross-reactivity | This antibody reacted only with the reference strains of Actinobacillus serotypes 5 |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab can be used for identification of serotype5 cells |
Curator ID | AA + AS |
Date of Curation | 02-02-2010 |
References | PMC1189361 |
PolysacDB ID | 1613 |
Carbohydrate Name | PTP1 antigen (Drugpedia) |
Carbohydrate Class | Glycoprotein |
Microbe | Encephalitozoon intestinalis (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Infected patient |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Indirect immunofluorescence, Western blotting, two-dimensional gel electrophoresis, and chemical deglycosylation |
Cross-reactivity | This antisera cross-reacted with E. hellem and E. cuniculi |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera was found to decrease the infectivity of E. intestinalis in vitro |
Curator ID | AA + AS |
Date of Curation | 23-09-2010 |
References | PMC1307029 |
PolysacDB ID | 1903 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pasteurella multocida serotype 1 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides. The O-antigen part consists of the following residues : -3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp-(1-. The core oligosaccharide part consists of the following residues : β-D-Galp-(1-->7)-D-gro-α-D-manHepp-(1-->6)-D-gro-α-D-manHepp-(1-->6)-β-D-Glcp-(1-->4)-L-gro [branched to L-gro-α-D-manHepp-(1-->2)-L-gro-α-D-manHepp-(1-->3)] and [branched to α-D-Glcp-(1-->6)] -α-D-manHepp-(1--/lipid A |
BCSDB Structure | N/A |
Proposed functions | LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | This antisera was specific to serotype 1 cells |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera may be useful in defining antigenic determinants of LPS. |
Curator ID | AA + AS |
Date of Curation | 25-11-2010 |
References | PMC271284 |
PolysacDB ID | 1904 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pasteurella multocida serotype 15 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides |
BCSDB Structure | N/A |
Proposed functions | LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | This antisera was specific to serotype 15 cells |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera may be useful in defining antigenic determinants of LPS. |
Curator ID | AA + AS |
Date of Curation | 25-11-2010 |
References | PMC271284 |
PolysacDB ID | 1905 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pasteurella multocida serotype 4 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides |
BCSDB Structure | N/A |
Proposed functions | LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | This antisera cross-reacted with serotype 12 cells |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera may be useful in defining antigenic determinants of LPS. |
Curator ID | AA + AS |
Date of Curation | 25-11-2010 |
References | PMC271284 |
PolysacDB ID | 1906 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pasteurella multocida serotype 12 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides |
BCSDB Structure | N/A |
Proposed functions | LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | This antisera cross-reacted with serotype 4 cells |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera may be useful in defining antigenic determinants of LPS. |
Curator ID | AA + AS |
Date of Curation | 25-11-2010 |
References | PMC271284 |
PolysacDB ID | 1999 |
Carbohydrate Name | Disaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The structure of the disaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-[5-->P-->(CH2)3NH2] |
BCSDB Structure | 5205 |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Keyhole limpet hemocyanin |
Conjugation Method | The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, phagocytosis assays and protection experiments |
Cross-reactivity | This antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera could passively protect mice against a 6B challenge |
Curator ID | AA + AS |
Date of Curation | 14-12-2010 |
References | PMC97953 |
PolysacDB ID | 2000 |
Carbohydrate Name | Trisaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The structure of the trisaccharide is -D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2] |
BCSDB Structure | 5205 |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Keyhole limpet hemocyanin |
Conjugation Method | The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, phagocytosis assays and protection experiments |
Cross-reactivity | This antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera could passively protect mice against a 6B challenge |
Curator ID | AA + AS |
Date of Curation | 14-12-2010 |
References | PMC97953 |
PolysacDB ID | 2001 |
Carbohydrate Name | Tetrasaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2] |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Keyhole limpet hemocyanin |
Conjugation Method | The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, phagocytosis assays and protection experiments |
Cross-reactivity | This antisera cross-reacted with capsular polysaccharide of 6A strains |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera could passively protect mice against a 6B and 6A challenge |
Curator ID | AA + AS |
Date of Curation | 14-12-2010 |
References | PMC97953 |
PolysacDB ID | 2071 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pseudomonas aeruginosa strain Fisher IT-3 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala] |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Alkali treated LPS |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, opsonophagocytic assays, animal protection studies and immunoblotting |
Cross-reactivity | This antisera was highly cross-reactive |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera had opsonophagocytic activity against LPS rough strains |
Curator ID | AA + AS |
Date of Curation | 07-01-2011 |
References | PMC172952 |
PolysacDB ID | 2072 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pseudomonas aeruginosa strain Fisher IT-4 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala] |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Alkali treated LPS |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, opsonophagocytic assays, animal protection studies and immunoblotting |
Cross-reactivity | This antisera was specific to strain IT-4 |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera had opsonophagocytic activity against LPS rough strains |
Curator ID | AA + AS |
Date of Curation | 07-01-2011 |
References | PMC172952 |
PolysacDB ID | 2073 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pseudomonas aeruginosa strain AK1401 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala] |
BCSDB Structure | 4396 |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Alkali treated LPS |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, opsonophagocytic assays, animal protection studies and immunoblotting |
Cross-reactivity | This antisera was highly cross-reactive |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera had opsonophagocytic activity |
Curator ID | AA + AS |
Date of Curation | 08-01-2011 |
References | PMC172952 |
PolysacDB ID | 2270 |
Carbohydrate Name | Y O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Heat-killed S. flexnerf bacteria serotype Y |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, FARR assay |
Cross-reactivity | The polyclonal rabbit anti-group antigen 3.4 antibodies reacted with the lb, 2a, 3b, 4a. 4b, and Y LPS. They did not bind to1a and 5a. In glycoconjugate ELISA's it bound to all saccharides especially abcd- and decasaccharide- BSA antigens |
Proposed epitopes | Antigen 3,4 seemed to be the epitope |
IEDB Epitope | 76696 |
Proposed Utility | This Mab would help in the delineation of the antigenic stucture of Y O polysaccharide |
Curator ID | AA + AS |
Date of Curation | 16-03-2011 |
References | 2438343 |
PolysacDB ID | 2323 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
BCSDB Structure | 3707 |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Heat killed cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | The highest titers seen were against serotype 3a and 3b LPSs |
Proposed epitopes | In S. flexneri serotype 3a and 3b LPSs there is no structural feature to which the type III antigen epitope can be assigned. Instead, the serotype specificity is a consequence of the addition of two group antigen epitopes to the basic structure in serotype 3a (7,8 and 6 epitopes) and of one group antigen epitope to the basic structure in serotype 3b (6 epitope). The α-D-glucose-(1-->3)-α-L-rhamnose was assigned as the determinant for group 7,8 antigen, and the 2-O-acetyl-α-L-rhamnose III structure was assigned as the determinant for group 6 antigen |
IEDB Epitope | 76815 |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 02-04-2011 |
References | PMC96386 |
PolysacDB ID | 2324 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)] (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
BCSDB Structure | N/A |
Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
Antigenic Nature used to produce antibodies | Heat killed cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgG |
Assay System | ELISA |
Cross-reactivity | This antisera strongly cross-reacted with S. flexneri LPS of serotypes 1a and 1b |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 02-04-2011 |
References | PMC96386 |
PolysacDB ID | 2325 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)] (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
BCSDB Structure | N/A |
Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
Antigenic Nature used to produce antibodies | Heat killed cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgA |
Assay System | ELISA |
Cross-reactivity | This antisera showed an extensive cross-reaction that included S. flexneri serotypes 1a, 1b, 2b, 4b, 5b, and Y |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | Cross-protection was observed among S. flexneri serotypes elicited by the S. flexneri 2a/3a vaccine |
Curator ID | AA + AS |
Date of Curation | 02-04-2011 |
References | PMC96386 |
PolysacDB ID | 2353 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Sinorhizobium meliloti strain RM41 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide) |
BCSDB Structure | N/A |
Proposed functions | LPS is an important surface antigen |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antisera was specific to the homologous immunizing strain |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species |
Curator ID | AA + AS |
Date of Curation | 13-04-2011 |
References | 10543844 |
PolysacDB ID | 2354 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Sinorhizobium fredii USDA205 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide) |
BCSDB Structure | N/A |
Proposed functions | LPS is an important surface antigen |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antisera was specific to the homologous immunizing strain |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species |
Curator ID | AA + AS |
Date of Curation | 13-04-2011 |
References | 10543844 |
PolysacDB ID | 2386 |
Carbohydrate Name | Streptococcal polysaccharide antigen eI (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus mutans strain MT703 serotype e (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The major fraction (eI) was identified as a polysaccharide composed of 37% glucose, 56% rhamnose, 5% protein, and 0.3% phosphorus, whereas the minor fraction (eII) contained 66% protein in addition to 10% glucose and 17% rhamnose |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Quantitative precipitin test and immunodiffusion and/or immunoelectrophoresis |
Cross-reactivity | This antisera cross-reacted with type E |
Proposed epitopes | Methyl-β-D-glucopyranoside residues seemed to be the predominant epitope. A β-linked glucose-glucose dimer is the predominant antigenic determinant of the e specificity |
IEDB Epitope | N/A |
Proposed Utility | This antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans |
Curator ID | AA + AS |
Date of Curation | 25-04-2011 |
References | PMC420845 |
PolysacDB ID | 2387 |
Carbohydrate Name | Streptococcal polysaccharide antigen eI (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus mutans strain K129 serotype E (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Quantitative precipitin test and immunodiffusion and/or immunoelectrophoresis |
Cross-reactivity | This antisera was specific to E1 |
Proposed epitopes | Methyl-β-D-glucopyranoside residues seemed to be the predominant epitope |
IEDB Epitope | N/A |
Proposed Utility | This antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans |
Curator ID | AA + AS |
Date of Curation | 25-04-2011 |
References | PMC420845 |
PolysacDB ID | 2388 |
Carbohydrate Name | Cell wall carbohydrate (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus GAS strain J-17 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Infected patients |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgG2 |
Assay System | ELISA |
Cross-reactivity | N/A |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae |
Curator ID | AA + AS |
Date of Curation | 25-04-2011 |
References | 8810949 |
PolysacDB ID | 2389 |
Carbohydrate Name | Cell wall carbohydrate (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus GAS strain J-18 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgG2 |
Assay System | ELISA |
Cross-reactivity | N/A |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae |
Curator ID | AA + AS |
Date of Curation | 26-04-2011 |
References | 8810949 |
PolysacDB ID | 2390 |
Carbohydrate Name | Type III polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus Group B strain M731 and M732 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | This polysaccharide has a mol wt of > 5 × 106 daltons. Its main chemical constituents are sialic acid (24%), galactose (25%), heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%) |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Capillary precipitin tests, immunodiffusion test |
Cross-reactivity | This antisera predominantly reacted with type III polysaccharide |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 26-04-2011 |
References | 55450 |
PolysacDB ID | 2409 |
Carbohydrate Name | Group B antigen (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus Group B strain M732 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | This polysaccharide had a molecular size > 5 x 106. Its main chemical constituents were sialic acid (24%), galactose (25%), a sugar tentatively identified as heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%) |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Formalin-killed whole cell vaccines |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Capillary precipitin tests, immunodiffusion tests, immunoelectrophoresis |
Cross-reactivity | This antisera cross-reacted with the TCA antigenas well as the EDTA-non pH titrated antigen and the group B antigen extracted with formamide from strain 090R or D136C |
Proposed epitopes | L-rhamnose was the immunodominant sugar for the formamide-extracted B antigen |
IEDB Epitope | N/A |
Proposed Utility | This antisera has helped determine that two antigens that react identically with each other and with similar specificities of antibody, yet with different immunodominant determinants |
Curator ID | AA + AS |
Date of Curation | 02-05-2011 |
References | 80423 |
PolysacDB ID | 2412 |
Carbohydrate Name | Phosphorylcholine (PC) (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial |
Antigenic Nature used to produce antibodies | ASRNKANDYTTEYSASVKGRFIVS [peptide1] |
Carrier Name | Bovine serum albumin |
Conjugation Method | BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, Opsonization and protection assays |
Cross-reactivity | N/A |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA |
Curator ID | AA + AS |
Date of Curation | 03-05-2011 |
References | 10992485 |
PolysacDB ID | 2413 |
Carbohydrate Name | Type V capsular polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The native type v polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-glucose and sialic acid in a molar ratio of 3:2:1:1. It consists of a repeating backbone of -->4)-α-D-Glcp-(1-->4)-α-D-Galp-(1-->4)-β-D-Glcp-(1--> with branching at 6th and 3rd positions |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA, Ouchterlony diffusion |
Cross-reactivity | Antibodies against the typeV capsuled o not cross-reactw ith other GBS capsular types and appear to recognize an epitope which is not as heavily dependent on the presenceo f the terminal side chain sialic acid residues as that of the type Ia, 11, and I11 GBS polysaccharides |
Proposed epitopes | β-D-Gal-(1-->4)-β-D-GlcNAc segmenot of the trisaccharide side chain, which is present in the core but not in the backbone polysaccharide, also contributes to the native epitope. The residues of the linear backbone and/or the glucopyranosyl side chain also form part of the antibody-binding epitope |
IEDB Epitope | N/A |
Proposed Utility | This antisera helped in the determination of immunodominant epitopes in Type V polysaccharide in group B Streptococcus |
Curator ID | AA + AS |
Date of Curation | 04-05-2011 |
References | 2016287 |
PolysacDB ID | 2421 |
Carbohydrate Name | Type 1 polysaccharide antigen (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus sanguis ST3 Serotype I (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The type I antigen was a polysaccharide composed of glucose, rhamnose, and N-acetylglucosamine in a molar ratio of 1.4:2.5:1.0. |
BCSDB Structure | N/A |
Proposed functions | The serotype I antigen may participate in the attachment of S. sanguis to tooth surfaces |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Quantitative precipitin inhibition tests, Immunodiffusion and immunoelectrophoresis |
Cross-reactivity | N/A |
Proposed epitopes | α-glucosidic linkage is the immunodeterminant of the type I antigen |
IEDB Epitope | N/A |
Proposed Utility | This antisera helped in determining the immunodominant region in the Type 1 polysaccharide |
Curator ID | AA + AS |
Date of Curation | 07-05-2011 |
References | PMC347986 |
PolysacDB ID | 2422 |
Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site |
BCSDB Structure | N/A |
Proposed functions | The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Tetanus toxoid |
Conjugation Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C. Adipic acid dihydrazide was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The CP-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and TT per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column |
Antibodies | Antisera |
Antibody type and class | IgG |
Assay System | Double immunodiffusion and capillary precipitation, opsonization assays |
Cross-reactivity | This antisera was quite specific and did not react with the structurally related pneumococcus type 14 polysaccharide |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | Conjugate-induced antibodies facilitated opsonization of group B streptococcus type III organisms. The type III-TT conjugate can be a potential vaccine for prevention of neonatal Group B Streptococcal diseases. |
Curator ID | AA + AS |
Date of Curation | 08-05-2011 |
References | 2407652 |
PolysacDB ID | 2426 |
Carbohydrate Name | Streptococcal A carbohydrate (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus Group A (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | This Carbohydrate is highly immunodominant |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Radioimmunoassay |
Cross-reactivity | N/A |
Proposed epitopes | The antigenic determinant was known as α hapten that had a estimated molecular weight of 1100 daltons and a rhamnose: glucosamine molar ratio of 6:1 |
IEDB Epitope | 76687 |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 10-05-2011 |
References | 4795261 |
PolysacDB ID | 2435 |
Carbohydrate Name | Trisaccharide part of Streptococcus pneumoniae type 3 polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Streptococcus pneumoniae Type 3 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Diphtheria toxin mutant (CRM197) |
Conjugation Method | The saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5 |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Enzyme-linked immunosorbent assay and in-vitro phagocytosis assay |
Cross-reactivity | N/A |
Proposed epitopes | β-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp |
IEDB Epitope | N/A |
Proposed Utility | This antisera provided full protection against challenge with pneumococcal type 3 bacteria |
Curator ID | AA + AS |
Date of Curation | 13-05-2011 |
References | PMC308892 |
PolysacDB ID | 2461 |
Carbohydrate Name | Synthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8 (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Salmonella spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Synthetic disaccharide abequose1-->3α-rhamnose |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Glycoconjugate |
Carrier Name | Bovine serum albumin |
Conjugation Method | N/A |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Immunofluorescence and co-agglutination (COA) |
Cross-reactivity | The antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci |
Curator ID | AA + AS |
Date of Curation | 23-05-2011 |
References | 373381 |
PolysacDB ID | 2463 |
Carbohydrate Name | Polysaccharide antigen (PS 02/725) (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Eubacterium saburreum strain 02/725 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | This polysaccharide consists of D-glycero-d-galacto-heptose and 6-deoxyheptose residues |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgG |
Assay System | Precipitin assay and complement binding assay |
Cross-reactivity | This antisera was specific to 02/725 polysaccharide |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 24-05-2011 |
References |
PolysacDB ID | 2464 |
Carbohydrate Name | Surface polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Eubacterium saburreum strain T18 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The purified antigen was a homopolysaccharide composed of D-glycero-D-galacto-heptose (Hep.) residues. The structure of the repeating unit in the polysaccharide was: -[-6)-[α Hep.furanosyl-(1-->4)]-β-Hep.pyranosyl- (1-->6)-[α-Hep.furanosyl-(1-->2), α-Hep.furanosyl-(1-->4)]-β- Hep.pyranosyl-(1-)4-->6)-β-Hep.pyranosyl-(1-->. No heptose residues were acetylated |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | Immunodiffusion tests |
Cross-reactivity | N/A |
Proposed epitopes | The immunodeterminant of the antigenic polysaccharide was D-glycero-D-galacto-heptofuranosyl residues as branched nonreducing terminals |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 25-05-2011 |
References | 1408355 |
PolysacDB ID | 2465 |
Carbohydrate Name | Polysaccharide antigen PS L13 (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Eubacterium saburreum L13 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | This polysaccharide contained ketohexose and glucose residues |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgG |
Assay System | Double diffusion experiments, Immunoelectrophoresis |
Cross-reactivity | N/A |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | Rabbit antiserum to E. saburreum L13 agglutinated the bacteria |
Curator ID | AA + AS |
Date of Curation | 25-05-2011 |
References | PMC347922 |
PolysacDB ID | 2469 |
Carbohydrate Name | Surface polysaccharide antigen (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Eubacterium saburreum strain L32 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The PS L32 polymer is built up of repeating trisaccharide units of galactose, ribose and dideoxyhexose |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgM |
Assay System | Precipitation tests, immunoelectrophoresis and complement fixation |
Cross-reactivity | N/A |
Proposed epitopes | The dideoxyhexose is the immunodominant sugar |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 27-05-2011 |
References | 670929 |
PolysacDB ID | 2470 |
Carbohydrate Name | Surface polysaccharide antigen (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Eubacterium saburreum strain T18 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | This antigen was composed of D-glycero-D-galacto-heptose |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | IgM |
Assay System | Immunoprecipitation, immunodiffusion |
Cross-reactivity | This antisera was specific to T18 antigen and did not cross-react with L44 antigen and T27 antigen |
Proposed epitopes | D-glycero-D-galacto-heptose constitutes part of the immunodeterminant of the antigenic polysaccharide |
IEDB Epitope | N/A |
Proposed Utility | The polysaccharide antigen from strain T18 is distinct from those previously reported for this species and may be useful in serotyping E. saburreum |
Curator ID | AA + AS |
Date of Curation | 28-05-2011 |
References | PMC260431 |
PolysacDB ID | 2495 |
Carbohydrate Name | O-polysaccharide [Terminal hexasaccharides] (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Vibrio cholerae O1 LPS serotype ogawa (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar is characterized by a 2-O-methyl group |
BCSDB Structure | 6642 |
Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
Antibodies | Antisera |
Antibody type and class | IgG and IgM |
Assay System | ELISA |
Cross-reactivity | N/A |
Proposed epitopes | The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain |
IEDB Epitope | 76824 |
Proposed Utility | This antisera showed protection |
Curator ID | AA + AS |
Date of Curation | 04-06-2011 |
References | 11920320 |
PolysacDB ID | 2496 |
Carbohydrate Name | O-polysaccharide [Terminal disaccharide] (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
BCSDB Structure | 6642 |
Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
Antibodies | Antisera |
Antibody type and class | IgM and IgG1 |
Assay System | ELISA |
Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
Proposed epitopes | N/A |
IEDB Epitope | 76775 |
Proposed Utility | This antisera was neither vibriocidal nor protective in the infant mouse cholera model |
Curator ID | AA + AS |
Date of Curation | 05-06-2011 |
References | PMC427411 |
PolysacDB ID | 2497 |
Carbohydrate Name | O-polysaccharide [Terminal trisaccharide] (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
BCSDB Structure | 6642 |
Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
Antibodies | Antisera |
Antibody type and class | IgM and IgG2 |
Assay System | ELISA |
Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
Proposed epitopes | N/A |
IEDB Epitope | 76775 |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 05-06-2011 |
References | PMC427411 |
PolysacDB ID | 2498 |
Carbohydrate Name | O-polysaccharide [Terminal tetrasaccharide] (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
BCSDB Structure | 6642 |
Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
Antibodies | Antisera |
Antibody type and class | IgM and IgG3 |
Assay System | ELISA, invivo protection assay |
Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
Proposed epitopes | N/A |
IEDB Epitope | 76775 |
Proposed Utility | This antisera helped in the epitope studies of O1 LPS |
Curator ID | AA + AS |
Date of Curation | 05-06-2011 |
References | PMC427411 |
PolysacDB ID | 2502 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Vibrio cholerae Non-O1 serogroup (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cell bacteria and purified LPS |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Antisera |
Antibody type and class | N/A |
Assay System | ELISA |
Cross-reactivity | This antisera was specific to V. cholerae had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera can be used for the detection of Vibrio cholerae non O1 strrains |
Curator ID | AA + AS |
Date of Curation | 08-06-2011 |
References | TUMS Journals |
Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |