PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1204
Carbohydrate NameSmooth Lipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella melitensis strain B115   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB StructureN/A
Proposed functionsLPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 05D01
Antibody type and classIgG1
Assay SystemELISA and immunoblotting
Cross-reactivityThis antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopesThis Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation07-03-2010
References1382111


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