PolysacDB ID | 2489 |
Carbohydrate Name | 35 and 50 KDa surface glycoconjugates (Drugpedia) |
Carbohydrate Class | Glycoprotein |
Microbe | Trypanosoma cruzi strain G (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A
|
BCSDB Structure | N/A |
Proposed functions | These glycoconjugates may play an important role in the metacyclic form-host cell association that initiates T. cruzi infection |
Antigenic Nature used to produce antibodies | Killed strain G metacyclic forms |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 10D8 |
Antibody type and class | N/A |
Assay System | ELISA, immunoblotiing, parasite invasion assay |
Cross-reactivity | 10D8 was found to react with metacyclic trypomastigotes from triatomine or from culture as well as with epimastigotes but not with tissue amastigotes or blood trypomastigotes |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The invasion of Vero cells by metacyclic forms of strains Tulahuen and G was inhibited 50 to 67% in the presence of 10D8. Mice that had passively received 10D8 before challenge with metacyclic forms, a considerable decrease in the parasitemia levels was observed |
Curator ID | AA + AS |
Date of Curation | 02-06-2011
|
References | PMC313336 |