PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1053
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli strain J5 (chemotype Rc)   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 11
Antibody type and classIgG3
Assay SystemELISA, Limulus assay, invivo protection assay
Cross-reactivityThis antibody ross-reacted with Salmonella minnesota strain R5
Proposed epitopesTerminal glucose residues
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation16-01-2010
References2455054


Entry No. - 2   [TOP]
PolysacDB ID2352
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSinorhizobium meliloti strain NRG185   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThere are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB StructureN/A
Proposed functionsLPS is an important surface antigen
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 11
Antibody type and classIgM
Assay SystemELISA and immunoblotting
Cross-reactivityThis Mab was highly cross-reactive recognizing most number of Shinorhizobium species. MAb 11 recognized only one major form (band) of R-LPS from the serogroup A strains, and it did not bind the S-LPS from any strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
Curator IDAA + AS
Date of Curation12-04-2011
References10543844


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036