PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1073
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli O18:K1:H7   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 14
Antibody type and classIgM
Assay SystemELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivityThis Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopesThe epitope was proposed to consist of the O-antigen
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation22-01-2010
ReferencesPMC261046


Entry No. - 2   [TOP]
PolysacDB ID2272
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeSelenomonas ruminantium HD4   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed Whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 14
Antibody type and classN/A
Assay SystemImmunofluorescence, immunoblotting and enzyme-linked immunosorbent assay
Cross-reactivityThis Mab cross-reacted with Selenomonas MP72 strain and M7 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be particularly valuable for in vitro assays of bacterial viability in mixed culture and for immunofluorescence screening of crude ruminal samples
Curator IDAA + AS
Date of Curation16-03-2011
ReferencesPMC184582


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