| PolysacDB ID | 2490 |
| Carbohydrate Name | Ssp-4 (amastigote surface glycoprotein) (Drugpedia) |
| Carbohydrate Class | Glycoprotein |
| Microbe | Trypanosoma cruzi amastigotes (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Ssp-4 is an amastigote-specific 84 KDa surface component defined by monoclonal antibody (MAb) 2C2, that was shown to be linked to the membrane by a GPI anchor. The epitope defined by MAb 2C2 is homogeneously distributed over the amastigote surface throughout the intracellular life cycle until it is gradually shed through a phospholipase-dependent mechanism during transformation into trypomastigote
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| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Mixtures of heat-inactivated intracellular and extracellular amastigotes |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 1D9 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunoblotting, immuno-electron microscopy and confocal immunofluorescence |
| Cross-reactivity | This Mab cross-reacts with membrane of amastigotes from 569, 588, 573, 587 and SC2005 isolates, similar to the G isolate. It also reacts with the CL isolate |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab reacted with cytoplasmic membrane-bound compartments of the amastigotes. This Mab specifically inhibited cell invasion by G and 588, but not CL strains |
| Curator ID | AA + AS |
| Date of Curation | 02-06-2011
|
| References | 9225447 |