PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1003
Carbohydrate NameGlucan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeMycobacterium tuberculosis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureGlucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB StructureN/A
Proposed functionsActivates leukocytes, stimulating their phagocytic, cytotoxic, and antimicrobial activities, including the production of reactive oxygen and nitrogen intermediates
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NamePseudomonas aeruginosa exoprotein A (rEPA)
Conjugation MethodThe polysaccharide was activated with CDAP. A 60-ml volume of CDAP (100 mg/ml of acetonitrile) was added to a solution of polysaccharide (2 ml, 10 mg of polysaccharide per ml of PFS) at room temperature. The pH was maintained at 5.8 to 6.0 for 30 s, and 60 ml of 0.2 M TEA was added to a pH of 7.0. The reaction was carried out for 2 min, and an equal volume of 0.8 M adipic acid dihydrazide [ADH] in 0.5 M NaHCO3 was added. This reaction was carried out for 2 h, and the pH was maintained at 8.0 to 8.5 with 0.1 N NaOH. The reaction mixture was dialyzed against PFS and passed through a column (3 by 46 cm) of P-10 in PFW. The void volume fractions were pooled, freeze-dried. ADH-derivatized polysaccharide (10 mg) was dissolved in PFS (2 ml). An equal weight of protein was added, and the pH was maintained at 5.1 to 5.5 with 0.1 M HCl. The reaction mixture was put on ice, EDAC was added to a final concentration of 0.05 M, and the pH was maintained at 5.1 to 5.5 for 4 h in 0.1 M HCl. The reaction mixtures were dialyzed against 0.2 M NaCl for 2 days with three changes of outer fluid and were passed through a column (1.5 by 90 cm) of Sepharose CL-6B in 0.2 M NaCl. The void volume fractions were stored at 3 to 88°C
AntibodiesMab 24c5
Antibody type and classIgG
Assay SystemELISA, Immunofluorescence
Cross-reactivityThis antibody cross-reacted with M. kansasii, BCG Pasteur, M. smegmatis, M. phlei, M. fortuitum, and M. avium
Proposed epitopesN\A
IEDB EpitopeN/A
Proposed UtilityThe antibody was used to study M. tuberculosis Glucan expression in vitro and in vivo
Curator IDAA + AS
Date of Curation01-01-2010
ReferencesPMC127896


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036