| PolysacDB ID | 2499 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae O1 LPS serotype ogawa 395 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 2D6 |
| Antibody type and class | IgA |
| Assay System | ELISA, agglutination assay, indirect immunofluorescence, EM immunogold labeling, invivo protection and immunoblotting |
| Cross-reactivity | This Mab is specific to V. cholerae Ogawa and did not cross-react with V. cholerae CA401, a virulent strain of the Inaba serotype |
| Proposed epitopes | The nonreducing terminal residue of Ogawa O-PS is the dominant determinant. The C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. [9446585] |
| IEDB Epitope | N/A |
| Proposed Utility | Neonatal mice bearing anti-lipopolysaccharide hybridoma backpack tumors were specifically protected against oral challenge |
| Curator ID | AA + AS |
| Date of Curation | 05-06-2011
|
| References | PMC258355 |