| PolysacDB ID | 1014 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
|
| BCSDB Structure | 125281 |
| Proposed functions | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugation Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Mab 2G8 |
| Antibody type and class | IgG2 |
| Assay System | Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay |
| Cross-reactivity | This antibody binds both C. neoformans and Candida albicans |
| Proposed epitopes | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo. |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010
|
| References | PMC2168274 |