PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 4
Entry No. - 1 [TOP]

PolysacDB ID	1038
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains.
BCSDB Structure	1686
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Mixture of suspension of cell envelop.
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3
Antibody type and class	N\A
Assay System	Western blotting, spot imunoassays, agglutination tests
Cross-reactivity	This antibody was cross-reactive with different strains of R.leguminosarum like R.biovars viciae, trifolli and phaseoli.
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N\A
Curator ID	AA + AS
Date of Curation	10-01-2010
References	PMC209711

Entry No. - 2 [TOP]

PolysacDB ID	1052
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3
Antibody type and class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross-reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed epitopes	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Entry No. - 3 [TOP]

PolysacDB ID	1484
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Histoplasma capsulatum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Disrupted yeast cell homogenate of Histoplasma capsulatum
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3
Antibody type and class	IgG1
Assay System	ELISA, immunoblotting and Solid-phase radioimmunoassay
Cross-reactivity	MAb 3 cross-reacted with B. dermatitidis, Paracoccidioides brasiliensis, Sporothrir schenckii, and Candida albicans
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	MAbs may prove useful in the development of more specific immunodiagnostic tests for histoplasmosis
Curator ID	AA + AS
Date of Curation	31-07-2010
References	PMC313195

Entry No. - 4 [TOP]

PolysacDB ID	2594
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Rhizobium leguminosarum biovar viciae strain 248 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment
Antigenic Nature used to produce antibodies	Peptidoglycan-outer membrane complex
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3
Antibody type and class	N/A
Assay System	ELISA, immunoblotting, Spot immunoassays, Agglutination tests
Cross-reactivity	This Mab was specific to the immunizing strain
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be a useful tool for studying the differentiation process from free-living bacteria to bacteroids
Curator ID	AA + AS
Date of Curation	13-07-2011
References	PMC209711

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036