| PolysacDB ID | 1663 |
| Carbohydrate Name | Glycopeptidolipid antigen (Drugpedia) |
| Carbohydrate Class | Glycolipid |
| Microbe | Mycobacterium avium complex serovar 4 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This antigen is composed of a glycosylated lipopeptide core, fatty acyl-D-phenylalanine-D-allothreonine-D-alanine-L-alaninyl-O-(3,4-di-O-methyl-α-L-rhamnopyranoside), to which a haptenic oligosaccharide is linked at the threonine substituent
|
| BCSDB Structure | 134896 |
| Proposed functions | This oligosaccharide unit is the source of the dominant immunogenicity of members of the M. avium complex, i.e., their characteristic agglutinability and type specificity |
| Antigenic Nature used to produce antibodies | Serovar 4-specific neoglycoprotein, 4-0-methyl-a-L-rhamnopyranosyl(1--> 4)-2-0 - methyl - alpha - L fucopyranosyl oxynonanoyl-bovine serum albumin (BSA) (neoantigen 4) along with whole washed M. avium complex serovar 4 cells and corresponding serovar 4 glycopeptidolipid antigens |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 32B8 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, Inhibition assays, dot blot |
| Cross-reactivity | This antibody cross-reacted with whole cells of serovar 4, acetylated-GPL-4, and GPL-4 |
| Proposed epitopes | 4-O-methyl-α-L-rhamnopyranosyl-(1-->4)-2-O-methyl-α-L-fucopyranosyl residues seemed to be a part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used for the identification and classification of M. avium isolates. This Mab can be applied directly to bacterial cultures, thus allowing easy, rapid identification of at least half of the M. avium isolates encountered in the clinical laboratory |
| Curator ID | AA + AS |
| Date of Curation | 01-10-2010
|
| References | PMC260783 |