PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2602
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCryptococcus neoformans Serotype D   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure136943
Proposed functionsThe capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameSheep erythrocytes
Conjugation MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 386
Antibody type and classN/A
Assay SystemELISA and Immunodiffusion
Cross-reactivityThis antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA
Proposed epitopesSince Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes
Curator IDAA + AS
Date of Curation15-07-2011
References2476401


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036