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| PolysacDB ID | 1115 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3E10 |
| Antibody type and class | IgG2b |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross-reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 03-02-2010 |
| References | 9116634 |
| PolysacDB ID | 2566 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Xanthomonas hyacinthi S148 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Crude cell wall preparation and purified fimbriae |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3E10 |
| Antibody type and class | IgG3 |
| Assay System | Immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy |
| Cross-reactivity | This Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas |
| Proposed epitopes | The O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes |
| Curator ID | AA + AS |
| Date of Curation | 01-07-2011 |
| References | 10473431 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |