| PolysacDB ID | 1106 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3E12 |
| Antibody type and class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
| Proposed epitopes | N/A
|
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 31-01-2010
|
| References | PMC170200 |