PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1097
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 4 (field isolate F6)   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7.
BCSDB Structure115743
Proposed functionsLPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodiesFormalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5B8
Antibody type and classIgG2b
Assay SystemWhole cell ELISA and slide agglutination tests
Cross-reactivityThis Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator IDAA + AS
Date of Curation28-01-2010
ReferencesPMC170200


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036