PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1095
Carbohydrate NameHigh molecular weight polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 4 (reference strain M62)   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5E4
Antibody type and classIgM
Assay SystemWhole cell ELISA and slide agglutination tests
Cross-reactivityThis Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab reacted with only 24.2 and 13.8% of homologous isolates, respectively, but detected 63% of the total 12 serotype 7 isolates. A minor reactivity was observed with other serotypes
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator IDAA + AS
Date of Curation28-01-2010
ReferencesPMC170200


Entry No. - 2   [TOP]
PolysacDB ID1387
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli J5   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-inactivated E. coli J5 Bacterial cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5E4
Antibody type and classIgG1
Assay SystemELISA, immunoblotting and RIA
Cross-reactivityThis antibody cross-reacted with E. coli J5, E. Coli O111:B4, S. minnesota R595, K. pneumoniae, S. marcescens, and P. Aeruginosa but did not cross-react with Staphylococcus aureus and Staphylococcus faecalis
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation06-07-2010
ReferencesPMC260436


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