| PolysacDB ID | 1166 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Bordetella pertusis 1414 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
|
| BCSDB Structure | N/A |
| Proposed functions | The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties |
| Antigenic Nature used to produce antibodies | B. pertusis Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 60.5 |
| Antibody type and class | IgM |
| Assay System | ELISA and Immunostaining |
| Cross-reactivity | This Mab did not cross-react with the B. pertusis variant strain A102 but cross-reacted with a B. bronchiseptica LPS |
| Proposed epitopes | The epitope was proposed to be terminal region of a distal trisaccharide GlcNAc-->2,3-NAcManA-->FucN(Ac)Me consisting of Nacetylated amino sugars. A next carbohydrate unit (2,3-NAcManA) may be partially involved in the recognized epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica |
| Curator ID | AA + AS |
| Date of Curation | 25-02-2010
|
| References | 8936324 |