PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1267
Carbohydrate NamePhosphomannan protein complex   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans NIH B-792   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed yeast-form cells of Candida albicans NIH B-792 strain
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 6A1
Antibody type and classIgM
Assay SystemELISA, Enzyme Immunoassay and Slide agglutination
Cross-reactivityThis antibody cross-reacted with NIH A-207 (serotype A) and J-1012 [serotype A] and C. tropicalis IFO 0587
Proposed epitopesThe epitope was proposed to contain β-1,2-linked oligomannosyl residues
IEDB EpitopeN/A
Proposed UtilityAntibodies to β- 1,2-linked oligomannosyl residues can be used as the serodiagnostic agent for detecting the C. albicans antigen(s) in sera and tissues of mammals with candidiasis.
Curator IDAA + AS
Date of Curation03-05-2010
References3280163


Entry No. - 2   [TOP]
PolysacDB ID2567
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 6A1
Antibody type and classIgG1
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


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