PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2027
Carbohydrate NameCore oligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed Whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 73.5
Antibody type and classIgG1
Assay SystemELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross-reactivityThe Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . Stutzeri and P. testosteroni but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab demonstrated opsonophagocytic killing of 06 subtype strains in the presence of human peripheral blood leukocytes and fresh human serum. This Mab also showed in vivo protection
Curator IDAA + AS
Date of Curation21-12-2010
References1588587


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