PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1039
Carbohydrate NameCapsular polysaccharides K5   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEscherichia coli   (NCBI Taxonomy)   (Drugpedia)
Basic StructureCapsular polysacchirides K5 is disaccharide with repeating unit 4-β-GlcUA(1-->4)-α-GlcNAc
BCSDB StructureN/A
Proposed functionsCapsular polysaccharides are responsible for pathogen`s virulence
Antigenic Nature used to produce antibodiesWhole formalinized E.coli cells.
Carrier NameN/A
Conjugation MethodN/A
AntibodiesMab 865
Antibody type and classIgM
Assay SystemIndirect ELISA, Immunoblot analysis
Cross-reactivityThis antibody was cross-reactive with ECA of E.coli and S.typhimurium strains.
Proposed epitopes4-β-GlcUA(1-->4)-α-GlcNAc
IEDB EpitopeN/A
Proposed UtilityReaction pattern of Mab with whole cell bacteria indicate that ECA and outer core in encapsulated bacteria are not readily accessible to antibodies.This suggests that these common envelop structures may not be useful antigens for vaccine development.
Curator IDAA + AS
Date of Curation11-01-2010
ReferencesPMC261975


Entry No. - 2   [TOP]
PolysacDB ID1067
Carbohydrate NameEnterobacterial common antigen   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeEscherichia coli C600   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a glycophospholipid built up by an amino sugar heteropolymer linked to an L-glycerophosphatidyl residue.
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole formalinized E. coli C600 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 865
Antibody type and classIgM
Assay SystemELISA and immunoblotting
Cross-reactivityThis antibody cross-reacted with purified ECA from S.montevideo and to E. coli Rl and R4 LPS, cross-reacted well with the K5 capsular polysaccharide
Proposed epitopesEpitope was proposed to contain 4-linked α-N-acetylglucosamine
IEDB EpitopeN/A
Proposed UtilityThis Mab may be used for the detection of ECA and K5 core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator IDAA + AS
Date of Curation20-01-2010
ReferencesPMC261975


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