PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1616
Carbohydrate NameLipooligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeMoraxella catarrhalis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo[ branched to Kdop-(2-?)]
BCSDB Structure22728
Proposed functionsMoracella lipooligosaccharide is a potential virulence factor
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 8E7
Antibody type and classIgG3
Assay SystemELISA, immunoblotting, Bacterial adherence assay, Bactericidal assay and immunoelectron microscopy
Cross-reactivityMAb 8E7 reacted with M. catarrhalis O35E, serotype A and C LOSs but not serotype B LOS
Proposed epitopesThe epitope recognized by MAb 8E7 is directed to a common sequence of either α-GlcNAc-(1-->2)-β-Glc-(1--> at the branch substituting position 4 of the trisubstituted Glc residue or a terminal tetrasaccharide α-Gal-(1-->4)-β-Gal-(1-->4)-α-Glc-(1-->2)-β-Glc-[1--> at the branch substituting position 6 of the trisubstituted Glc residue
IEDB EpitopeN/A
Proposed UtilityThis Mab had had bactericidal activity. Passive immunization with Mab 8E7 could significantly enhance the clearance of strain O35E from mouse lungs in an aerosol challenge mouse model
Curator IDAA + AS
Date of Curation23-09-2010
ReferencesPMC98028


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