PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID2508
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae 01 serogroup   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure6642
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesKilled Vaccine consisting of V. cholerae O1 Inaba (ATCC 14033) and Ogawa (ATCC 14035) cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab A6
Antibody type and classIgG3
Assay SystemEnzyme-linked immunosorbent assay (ELISA), immunofluorescence, Immunoelectron microscopy and the slide agglutination test
Cross-reactivityThis Mab reacted with both Inaba and Ogawa serovars (A antigen) and was specific to O1 serotype
Proposed epitopesThe epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
IEDB EpitopeN/A
Proposed UtilityThis Mab produced discernible agglutination within 1 min with each of the 37 01 whole-cell suspensions tested
Curator IDAA + AS
Date of Curation10-06-2011
ReferencesPMC266720


Entry No. - 2   [TOP]
PolysacDB ID2560
Carbohydrate NameExopolysaccharide xanthan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeXanthomonas campestris pv. campestris 646   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt consists of a β1-->4 glucose polymer, i.e. a cellulose backbone, with β1-->3 substituted trisaccharide side-chains of (mannose-β1-->4 glucuronic acid-α-1-->2-mannose-β-1-->3)- on alternate glucose residues. The side-chain is variably acylated. The inner mannose may have an O-6 acetyl group and the terminal mannose a 4,6- bound pyruvate ketal
BCSDB StructureN/A
Proposed functionsThe exopolysaccharide xanthan from Xanthomonas campestris is an interesting carbohydrate polymer, with striking rheological properties which make it useful as a food additive (gelling agent) and also as a potential vehicle in enhanced oil recovery
Antigenic Nature used to produce antibodiesXanthan
Carrier NameNil
Conjugation MethodNil
AntibodiesMab A6
Antibody type and classIgM
Assay SystemELISA
Cross-reactivityThis Mab was specific to Xanthan and did not cross-react with Cellulose
Proposed epitopesThe specificity was mainly against the side-chain of xanthan. This Mab was assumed to be directed against the nonsubstituted trisaccharide with the inner mannose-glucuronic acid being immunodominant
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation28-06-2011
References2621443


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036