| PolysacDB ID | 2605 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
|
| BCSDB Structure | 136943 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Keyhole limpet hemocyanin |
| Conjugation Method | Forty milligrams of crude polysaccharide was dissolved in 4 ml of physiological saline solution (PSS). One milliliter of 1% cyanuric chloride in dimethylformamide was added to the polysaccharide solution and stirred for 1 h at 48°C. KLH in PSS (100 mg/2ml) was added and stirred for 1 h at 258°C, and the solution was then incubated at 48C for 18 h. The solution was dialyzed against water for 30 h and centrifuged. The protein concentration in the supernatant was adjusted to 2 mg/ml, 1/9 volume of 10% aluminum potassium sulfate solution was added, and the pH was adjusted to 6.5 with 10% sodium carbonate. The precipitated antigen was removed by centrifugation and resuspended in PSS |
| Antibodies | Mab CRND8 |
| Antibody type and class | IgM |
| Assay System | ELISA and CSA[Cell Slide Agglutination] |
| Cross-reactivity | This antibody cross-reacted with Serotype A and D serotypes |
| Proposed epitopes | O-acetylated glucurunoxylomannan |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011
|
| References | PMC170253 |