PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2563
Carbohydrate NameExopolysaccharide xanthan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeXanthomonas campestris pv. campestris 649   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt consists of a β1-->4 glucose polymer, i.e. a cellulose backbone, with β1-->3 substituted trisaccharide side-chains of (mannose-β1-->4 glucuronic acid-α-1-->2-mannose-β-1-->3)- on alternate glucose residues. The side-chain is variably acylated. The inner mannose may have an O-6 acetyl group and the terminal mannose a 4,6- bound pyruvate ketal
BCSDB StructureN/A
Proposed functionsThe exopolysaccharide xanthan from Xanthomonas campestris is an interesting carbohydrate polymer, with striking rheological properties which make it useful as a food additive (gelling agent) and also as a potential vehicle in enhanced oil recovery
Antigenic Nature used to produce antibodiesXanthan
Carrier NameNil
Conjugation MethodNil
AntibodiesMab D3
Antibody type and classIgG2a
Assay SystemELISA
Cross-reactivityThis Mab was specific to Xanthan and did not cross-react with Cellulose
Proposed epitopesThe specificity was mainly against the side-chain of xanthan. This Mab was assumed to be directed against the nonsubstituted trisaccharide with the inner mannose-glucuronic acid being immunodominant
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation29-06-2011
References2621443


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