| PolysacDB ID | 1901 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Pasteurella haemolytica serotype 1 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides. The O-antigen part consists of the following residues : -3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp-(1-. The core oligosaccharide part consists of the following residues : β-D-Galp-(1-->7)-D-gro-α-D-manHepp-(1-->6)-D-gro-α-D-manHepp-(1-->6)-β-D-Glcp-(1-->4)-L-gro [branched to L-gro-α-D-manHepp-(1-->2)-L-gro-α-D-manHepp-(1-->3)] and [branched to α-D-Glcp-(1-->6)] -α-D-manHepp-(1--/lipid A
|
| BCSDB Structure | N/A |
| Proposed functions | LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes |
| Antigenic Nature used to produce antibodies | P. hemolytica extract |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab H6B5 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunoblotting |
| Cross-reactivity | This MAb had intense cross-reactivity among P. haemolytica serotypes 1, 5, 6, 7, 8, and 12 and less reactivity with serotypes 4 and 14 but did not react with LPS from any other gram negative bacteria tested |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb to the LPS of P. haemolytica could be used to help further characterize the role of LPS in the pneumonic pasteurellosis disease process, including common binding sites and epitopes within the LPS molecule. It could be used in a screening kit, such as latex bead agglutination, to rapidly determine the presence of P. haemolytica in pneumonic lesions |
| Curator ID | AA + AS |
| Date of Curation | 25-11-2010
|
| References | PMC266479 |