| PolysacDB ID | 2579 |
| Carbohydrate Name | Lipid-A (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall
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| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM37 |
| Antibody type and class | IgM |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 07-07-2011
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| References | PMC178005 |