PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1910
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed functionsYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab M6C
Antibody type and classIgM
Assay SystemInhibition ELISA, double immunodiffusion
Cross-reactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation26-11-2010
References18991095


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