| PolysacDB ID | 2319 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 1b (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β[branched to α-D-Glcp(1-->4)]-D-GlcpNAc-(1--> |
| BCSDB Structure | 108641 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Heat killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF I |
| Antibody type and class | IgG1 |
| Assay System | Immunoblotting, enzyme-linked immunosorbent assay (ELISA), hemagglutination, and coagglutination |
| Cross-reactivity | The type I O antigen-specific antibodies were shown to bind exclusively to S. flexneri serotypes la and lb in ELISA |
| Proposed epitopes | The α-D-Glcp-(1-->4)-β-D-GlcpNAc disaccharide in the repeating unit of the O-antigen is the immunodominant region to which these antibodies bind |
| IEDB Epitope | 76664 |
| Proposed Utility | This Mab can be used for detection and identification of shigella flexneri species |
| Curator ID | AA + AS |
| Date of Curation | 01-04-2011
|
| References | 2424839 |