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PolysacDB ID | 2265 |
Carbohydrate Name | Y O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Heat-killed S. flexnerf bacteria serotype Y |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab MASF Y-1 |
Antibody type and class | N/A |
Assay System | ELISA, FARR assay |
Cross-reactivity | This Mab bound only to LPS of serotypes 4a and Y. In the glycoconjugate ELISA, MASF Y-1 bound only to the octasaccharide-(cdabcdab') and the decasaccharide-(abcdabcdab') BSA conjugate |
Proposed epitopes | This Mab bound to intrachain determinants |
IEDB Epitope | 76764 |
Proposed Utility | This Mab would help in the delineation of the antigenic stucture of Y O polysaccharide |
Curator ID | AA + AS |
Date of Curation | 15-03-2011 |
References | 2438343 |
PolysacDB ID | 2281 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
BCSDB Structure | 3226 |
Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab MASF Y-1 |
Antibody type and class | IgM |
Assay System | ELISA |
Cross-reactivity | MASF Y-1 bound to 4a and Y LPS |
Proposed epitopes | N/A |
IEDB Epitope | 76891 |
Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
Curator ID | AA + AS |
Date of Curation | 17-03-2011 |
References | 2437036 |
Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |