PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID2268
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-4
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityMASF Y-4 bound to LPS of serotypes 2a. 3b, 4a, 4b, 5a, and Y
Proposed epitopesThe terminal nonreducing end of the O-polysaccharide complementary to the tetrasaccharide repeating unit
IEDB Epitope76696
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 2   [TOP]
PolysacDB ID2284
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri serotype Y   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3226
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-4
Antibody type and classIgM
Assay SystemELISA
Cross-reactivityMASF Y-4 antibodies bound to 2a, 3b, 4a, 4b, 5a, and Y LPSs
Proposed epitopesN/A
IEDB Epitope76891
Proposed UtilityThis Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria
Curator IDAA + AS
Date of Curation18-03-2011
References2437036


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036