PolysacDB ID | 2058 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pseudomonas syringae IMV K-1025 pv. Atrofaciens (NCBI Taxonomy) (Drugpedia) |
Basic Structure | O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
|
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab Ps1b |
Antibody type and class | IgM |
Assay System | Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting |
Cross-reactivity | " P. syringae strains with pathovars glycinea, japonica, striafaciens, atrofaciens and 2 P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1c" |
Proposed epitopes | The epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose |
IEDB Epitope | N/A |
Proposed Utility | This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens |
Curator ID | AA + AS |
Date of Curation | 02-01-2011
|
References | PMC178531 |