PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1369
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed functionsLPS represents one of the major surface antigens of this microorganism
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-2
Antibody type and classIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross-reactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed epitopesThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation30-06-2010
ReferencesPMC256998


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036