| PolysacDB ID | 1582 |
| Carbohydrate Name | N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a homopolymer of α(2-->8) sialic acid
|
| BCSDB Structure | N/A |
| Proposed functions | This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature |
| Antibodies | Mab Seam 13 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, flow cytometry and indirect immunofluorescence |
| Cross-reactivity | This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit and human complement |
| Curator ID | AA + AS |
| Date of Curation | 14-09-2010
|
| References | 9590252 |