PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1605
Carbohydrate NameN-propionyl substituted Neisseria meningitidis group B capsular polysaccharide    (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeNeisseria meningitidis Serogroup B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a homopolymer of α(2-->8) sialic acid
BCSDB StructureN/A
Proposed functionsThis capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodThe oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
AntibodiesMab Seam 3
Antibody type and classIgG2b
Assay SystemELISA, flow cytometry and indirect immunofluorescence
Cross-reactivityThis Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
Curator IDAA + AS
Date of Curation21-09-2010
References9590252


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