| PolysacDB ID | 1012 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1-
|
| BCSDB Structure | 6237
|
| Proposed functions | Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8°C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody type and class | Mainly IgG, particularly IgG1 and IgG3 |
| Assay System | Double immunodiffusion, capillary precipitation, ELISA |
| Cross-reactivity | N/A |
| Proposed epitopes | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010
|
| References | PMC258520 |