| PolysacDB ID | 2321 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 1b (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β[branched to α-D-Glcp(1-->4)]-D-GlcpNAc-(1--> |
| BCSDB Structure | 108641
|
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Heat killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF 6-1 |
| Antibody type and class | IgG1 |
| Assay System | Immunoblotting, enzyme-linked immunosorbent assay (ELISA), hemagglutination, and coagglutination |
| Cross-reactivity | This Mab bound to LPSs of S. flexneri serotypes lb, 3a, 3b, and 4b |
| Proposed epitopes | These LPSs have one antigenic determinant i.e group 6 antigen. It is therefore likely that the structure responsible for binding is the O-acetyl group on C-2 of rhamnose III |
| IEDB Epitope | 76664 |
| Proposed Utility | This Mab can be used for detection and identification of shigella flexneri species |
| Curator ID | AA + AS |
| Date of Curation | 01-04-2011
|
| References | 2424839 |