| PolysacDB ID | 2422 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site
|
| BCSDB Structure | N/A |
| Proposed functions | The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C. Adipic acid dihydrazide was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The CP-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and TT per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column |
| Antibodies | Antisera |
| Antibody type and class | IgG |
| Assay System | Double immunodiffusion and capillary precipitation, opsonization assays |
| Cross-reactivity | This antisera was quite specific and did not react with the structurally related pneumococcus type 14 polysaccharide |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Conjugate-induced antibodies facilitated opsonization of group B streptococcus type III organisms. The type III-TT conjugate can be a potential vaccine for prevention of neonatal Group B Streptococcal diseases. |
| Curator ID | AA + AS |
| Date of Curation | 08-05-2011
|
| References | 2407652 |