| PolysacDB ID | 2642 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This polysaccharide is a high m.w. polymer with a linear α-(1-->3)-linked mannose backbone that is substituted with non-reducing D-xyIosyal and D-glucosyluronic acid groups. O-Acetylation varies with the serotype. Type D is the most heavily O-acetylated
|
| BCSDB Structure | 136943
|
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Methylated bovine serum albumin |
| Conjugation Method | A saline solution containing 500 pg of cryptococcal polysaccharide was mixed with 500 pg aqueous methylated bovine serum albumin. The volume was brought to 2 ml with sterile distilled water |
| Antibodies | Polysera |
| Antibody type and class | IgG |
| Assay System | Antiserum to untreated cryptococcal polysaccharide was enriched for O-acetyl and carboxyl specific antibody respectively. The antisera was then used in ouchterlony double diffusion. |
| Cross-reactivity | N/A |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | O-acetyl and carboxyl groups are strong and distinct physicochemical determinants on cryptococcal polysaccharide. The enriched antiserum displayed a level of opsonic activity that was similar to non-enriched antiserum. This showed that neither O-acetyl nor carboxyl groups were responsible for inhibition of phagocytosis by cryptococcal polysaccharide |
| Curator ID | AA + AS |
| Date of Curation | 30-07-2011
|
| References | 15271943 |