PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 4

Entry No. - 1   [TOP]
PolysacDB ID1122
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 1   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-polsaccharide structure is as follows :
--6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage.
BCSDB StructureN/A
Proposed functionsLPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodiesFormalin-fixed A. pleuropneumoniae serotype 1 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolysera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antibody cross-reacted with serotypes 9 and 11
Proposed epitopesThe epitope was deduced to contain the terminal nonreducing -D-Glc NAc residues
IEDB EpitopeN/A
Proposed UtilityThis antisera not only helped in serotyping analysis but also helped in deducing the epitopes recognized
Curator IDAA + AS
Date of Curation06-02-2010
ReferencesPMC206369


Entry No. - 2   [TOP]
PolysacDB ID1124
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 1   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolysera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antibody was specific to serotype 1 only
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation07-02-2010
ReferencesPMC206369


Entry No. - 3   [TOP]
PolysacDB ID1137
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 1   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesLMab-1
Antibody type and classIgM
Assay SystemELISA inhibition tests
Cross-reactivityLMAb-1 reacted to only a serotype 1, strain 4074
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be useful for serotyping studies
Curator IDAA + AS
Date of Curation13-02-2010
References1382625


Entry No. - 4   [TOP]
PolysacDB ID1138
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 1   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-polsaccharide structure is as follows :
--6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage.
BCSDB StructureN/A
Proposed functionsLPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesLMab-5
Antibody type and classIgM
Assay SystemELISA inhibition tests
Cross-reactivityIMAb-5 reacted with serotype 1, 9 and 11 strains at the same degree and its epitope was found to be located on O-polysaccharide of serotype 1, 9 or 11 lipopolysaccharide (LPS)
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be useful for serotyping studies
Curator IDAA + AS
Date of Curation13-02-2010
References1382625


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