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PolysacDB ID | 1096 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 7F5 |
Antibody type and class | IgG2a |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 28-01-2010 |
References | PMC170200 |
PolysacDB ID | 1097 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 5B8 |
Antibody type and class | IgG2b |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 28-01-2010 |
References | PMC170200 |
PolysacDB ID | 1098 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 7E6 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 28-01-2010 |
References | PMC170200 |
PolysacDB ID | 1099 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 5G4 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 29-01-2010 |
References | PMC170200 |
PolysacDB ID | 1100 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 4f1 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 29-01-2010 |
References | PMC170200 |
PolysacDB ID | 1101 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 4B8 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 29-01-2010 |
References | PMC170200 |
PolysacDB ID | 1102 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 7E10 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 29-01-2010 |
References | PMC170200 |
PolysacDB ID | 1103 |
Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 5C5 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 30-01-2010 |
References | PMC170200 |
PolysacDB ID | 1104 |
Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 1E10 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 30-01-2010 |
References | PMC170200 |
PolysacDB ID | 1105 |
Carbohydrate Name | Unknown carbohydrate (Drugpedia) |
Carbohydrate Class | Glycoprotein |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 6F7 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 31-01-2010 |
References | PMC170200 |
PolysacDB ID | 1106 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 3E12 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 31-01-2010 |
References | PMC170200 |
PolysacDB ID | 1107 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
BCSDB Structure | 115743 |
Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 7C3 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 31-01-2010 |
References | PMC170200 |
PolysacDB ID | 1108 |
Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 5H7 |
Antibody type and class | IgM |
Assay System | Whole cell ELISA and slide agglutination tests |
Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
Curator ID | AA + AS |
Date of Curation | 31-01-2010 |
References | PMC170200 |
Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |